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  Newsletter April 2013

 

April 2013
Runs 37, B15 and H3 were accomplished January to April 2013. About 300 laboratories participated in one or more modules. A very short summary of the tests is given below. Click on the epitope name to see the complete general assessment results for each marker, including recommendations for clone selection, epitope retrieval and control tissues.

Run 37 (General module)

CD3: 219 labs participated. 92% of the stains were sufficient, 66% optimal. Many clones can be used, and especially the Ready-To-Use systems from Dako, Leica and Ventana gave a high proportion of optimal results. HIER is mandatory.

CD45: 205 labs participated. 82% of the stains were sufficient but only 56% optimal. Correct selection of control tissue (liver) and identification of the critical stain quality indicator in the control tissue (Kupffer cells) is the basis for optimization. Efficient HIER is mandatory.

CD56: 196 labs participated. 81% of the stains were sufficient but only 49% optimal. Too low concentration of the primary antibody and less successful performance of the mAb clones 123C3 and 123C3.D5 on the Ventana BenchMark platform are the main reasons for suboptimal results. Ventana users should change to rmAb clone MRQ-42.

Carcinoembryonic antigen (CEA): 190 labs participated. Only 59% of the stains were sufficient and only 20% optimal. mAb clones TF3H8-1 and 12-140-10 constantly give insufficient results and should be discarded. mAb clone II-7does not work properly on the Ventana platform, Ventana used should change to  mAb clones CEA31 or COL-1. A further 20 labs submitted stains using inappropriate polyclonal Abs, which should not be used for CEA due to heavy cross reaction with other CEA-like proteins.

Synaptophysin (SYP): 102 labs participated. Only 58% of the stains were sufficient and only 19% optimal. Too dilute Abs, inefficient HIER, and use of the poor clone SY38 are the main reasons for suboptimal stains. mAb clone 27G12 which works well with mots systems perform poorly on the Ventana platform. Ventana users should change to rmAb clones MRQ-40 or SP11.

Run B15 (Breast cancer IHC module)

Estrogen receptor (ER): 241 laboratories participated, 85 % were sufficient but only 58% optimal. Main reason for suboptimal staining is as usual too dilute Ab and insufficient HIER. Clone 6F11 gave a positive staining reaction in one of the cores found negative with all other Abs. The reason for this is stil under investigation.

HER-2 IHC: 272 laboratories participated in this assessment. 90 % achieved a sufficient mark. The performance of FDA approved kits is consistently better than tha tof in-house validated systems.

Run H3 (HER-2 ISH module)

HER-2 ISH: 102 labs participated with BRISH and 30 with FISH. 81% of the BRISH stains were sufficient. Inappropriate epitope retrieval was the major cause of suboptimal reactions. Scoring consensus was slightly higher for FISH than for BRISH due to one of the non-amplified carcinoma cores scored as amplified by 12/90 labs using BRISH.

16th April 2013
Mogens Vyberg
Scheme director

 

 
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Last update 16-04-2013