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Multi tissue block for control

1. Paraffin block containing a tissue, e.g., lung adenocarcinoma, with a previously determined level of expression of an epitope, e.g., cytokeratin 7.

 

2. A skin punch biopsy needle is used to cut out representative tissue. The size of the needle can vary from 2 to 6 mm depending on personal preference, the size of the original sample in the block, and the number of pieces to be included in the multi-tissue block. The skin punch biopsy needle has to be hollow so that one can use a wooden or metal probe to push out sampled tissue.

 

3. Some blocks may be extensively sampled to prepare multiple multi-tissue blocks. However, substantial amount of tissue representative of the lesion should be preserved for the archives.

 
4. If more than one sample is prepared at the time, tissue fragments are stored in appropriate containers labelled with the pathology number, diagnosis, antigen that is expressed and the level of expression. 5. Samples are combined in order to include tissues with strong, weak, and if possible, intermediate expression of antigen. 6. Variable CK7 expression in the positive control can be seen on this slide even macroscopically.
 
ET/AS

Last update 21-09-2005