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Chromogen

Enhance the stain signal

The most used chromogens are Diaminobenzidine (DAB) and Aminoethylcarbazole (AEC). The advantage of DAB is the possibility of enhancement using imidazol and a metal salt, e.g., copper sulphate, thereby increasing the sensitivity. Another advantage is that DAB allows hydrophobic embedding. A new red chromogen, NovoRed, is almost as sensitive as DAB and also allows hydrophobic embedding. 

A change from AEC to an enhanced DAB has in our laboratory given a four fold enhancement on the average.

MV/SN/AS

Last update 21-09-2005