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Home ■ Participation ■ Assessments ■ Epitopes ■ Protocols ■ Techniques ■ Links |
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Tissue handling ■
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Endogenous biotin Primary antibody ■ Visualization ■ Chromogene ■ Controls ■ Recommended control tissue |
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Chromogen |
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Enhance the stain signal The most used chromogens are Diaminobenzidine (DAB) and Aminoethylcarbazole (AEC). The advantage of DAB is the possibility of enhancement using imidazol and a metal salt, e.g., copper sulphate, thereby increasing the sensitivity. Another advantage is that DAB allows hydrophobic embedding. A new red chromogen, NovoRed, is almost as sensitive as DAB and also allows hydrophobic embedding. A change from AEC to an enhanced DAB has in our laboratory given a four fold enhancement on the average. |
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