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Home ■ Participation ■ Assessments ■ Epitopes ■ Protocols ■ Techniques ■ Links |
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Tissue handling
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Section handling
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Epitope retrieval
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Endogenous biotin Primary antibody ■ Visualization ■ Chromogene ■ Controls ■ Recommended control tissue |
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Techniques |
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Optimizing the IHC protocols Immunohistochemistry is technically complex, and no aspect of this complexity can be ignored, from the moment of collecting the specimen to issuance of the final report (Taylor CR. Arch Pathol Lab Med 2000; 124:945).
The techniques pages (see Links above) give you some hints regarding optimizing the various steps in your protocol. The 'Controls' page illustrate a method of producing multi-tissue blocks. Also see the table Recommended control tissue. |
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