|
Protocol provided by |
|
| |
laboratory |
Pathology |
| |
hospital/institution |
Seinäjoki Central Hospital, Finland |
| |
e-mail address |
antero.laasonen@epshp.fi |
|
Primary antibody |
|
|
|
clone |
Ks 20.8 |
|
|
producer |
DakoCytomation |
|
|
product no., lot no. |
M 7019, 89 |
|
|
dilution |
1:100 |
|
|
diluent buffer and additive(s) |
DakoCytomation ChemMate™ diluent |
|
|
incubation time/temp. |
25 min./RT |
| Epitope retrieval, HIER |
|
| |
device |
autoclave |
| |
buffer type, pH |
Tris-EDTA/EGTA pH 9 |
| |
max. temp./max. temp. time |
105ºC/NS |
| |
total heating time/resting time |
5 min./15 min. |
| Epitope retrieval, proteolysis |
|
| |
protease, type |
proteinase K |
| |
dilution |
NS |
| |
incubation time/temp. |
5 min./RT |
| Visualization system |
|
| |
method |
polymer conjugate, PowerVision™+ |
| |
producer, product no. |
ImmunoVision, DPVB-150HRP |
| |
incubation time/temp. |
25 min./21ºC |
| Chromogen |
|
| |
type |
DAB+ |
| |
producer, product no. |
DakoCytomation, K 3468 |
| |
incubation time/temp. |
3 x 5 min./NS |
| |
enhancement, type |
0,5 % CuSO4 |
| Immunostainer |
|
| |
type |
TechMate 500 + |
| |
|
|
| Abbreviations |
|
| |
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature |
|
Disclaimer |
|
| |
NordiQC makes every
attempt to provide accurate and up-to-date
information, yet NordiQC does not make any claim or
warranty regarding the accuracy of the provided
information nor does it represent that the contents of
the web site and protocols reflect the most recent
developments in immunohistochemistry at any point in
time. |
|
