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Protocol provided by |
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| |
laboratory |
Antomical Pathology/Immunohistochemistry |
| |
hospital/institution |
Westmead |
| |
e-mail address |
jane.milliken@swahs.health.nsw.gov.au |
|
Primary antibody |
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|
clone |
NB10 |
|
|
producer |
Cell Marque |
|
|
product no., lot no. |
321M-16, 29060 |
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|
dilution |
1:50 |
|
|
diluent buffer and additive(s) |
VP Monet Blue, Biocare |
|
|
incubation time/temp. |
32 min./36°C |
| Epitope retrieval, HIER |
|
| |
device |
BenchMark ULTRA, Ventana |
| |
buffer type, pH |
Cell Conditioning 1, pH 8.5 |
| |
warm-up/heating max/resting time |
8 min./64 min./8 min. |
| |
maximum heating temp. |
95ºC |
| Visualization system |
|
| |
method |
2-step multimer conjugate |
| |
producer, product no. |
Ventana, 760-500 |
| |
incubation time/temp. |
8 min./36ºC |
| Chromogen |
|
| |
type |
DAB |
| |
producer, product no. |
Ventana, 760-500 |
| |
incubation time/temp. |
8 min./36ºC |
| |
enhancement, type |
NS |
| Immunostainer |
|
| |
type |
BenchMark ULTRA, Ventana |
| |
|
|
| Comments |
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| |
|
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| Abbreviations |
|
| |
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature |
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Disclaimer |
|
| |
NordiQC makes every
attempt to provide accurate and up-to-date
information, yet NordiQC does not make any claim or
warranty regarding the accuracy of the provided
information nor does it represent that the contents of
the web site and protocols reflect the most recent
developments in immunohistochemistry at any point in
time. |
|
