|
Protocol provided by |
|
| |
laboratory |
Patologi afd. |
| |
hospital/institution |
Næstved Sygehus, Sygehus Syd |
| |
e-mail address |
mibz@regionsjaelland.dk |
|
Primary antibody |
|
|
|
clone |
LE-CD19 |
|
|
producer |
Dako |
|
|
product no., lot no. |
M7296, 00063795 |
|
|
dilution |
1:50 |
|
|
diluent buffer and additive(s) |
Antibody diluent, K8006 Dako |
|
|
incubation time/temp. |
30 min./RT |
| Epitope retrieval, HIER |
|
| |
device |
PT Link, Dako |
| |
buffer type, pH |
TRS pH 9 (3-in-1) (Dako) |
| |
warm-up/heating max/resting time |
8 min./20 min./10 min. |
| |
maximum heating temp. |
97ºC (start 85ºC) |
| Visualization system |
|
| |
method |
3-step polymer conjugate |
| |
producer, product no. |
Dako, Envision Flex+, K8002 |
| |
incubation time/temp. |
20 min./30min./RT |
| Chromogen |
|
| |
type |
DAB |
| |
producer, product no. |
Dako Flex, SM803 |
| |
incubation time/temp. |
10 min./RT |
| |
enhancement, type |
NS |
| Immunostainer |
|
| |
type |
Autostainer Link 48, Dako |
| |
|
|
| Comments |
|
| |
|
|
| Abbreviations |
|
| |
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature |
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Disclaimer |
|
| |
NordiQC makes every
attempt to provide accurate and up-to-date
information, yet NordiQC does not make any claim or
warranty regarding the accuracy of the provided
information nor does it represent that the contents of
the web site and protocols reflect the most recent
developments in immunohistochemistry at any point in
time. |
|
