|
Protocol provided by |
|
| |
laboratory |
Pat/cyt |
| |
hospital/institution |
Aleris Medilab, Täby, Sweden |
| |
e-mail address |
cecilia.winge@aleris.se |
|
Primary antibody |
|
|
|
clone |
SP4 |
|
|
producer |
Biocare Medical |
|
|
product no., lot no. |
CRM307, 51409 |
|
|
dilution |
1:100 |
|
|
diluent buffer and additive(s) |
Renoir Red |
|
|
incubation time/temp. |
30 min./RT |
| Epitope retrieval, HIER |
|
| |
device |
Pressure cooker |
| |
buffer type, pH |
BORG Biocare, PH 9-10 |
| |
warm-up/heating max/resting time |
10 min./5 min./30 min. |
| |
maximum heating temp. |
121ºC |
| Visualization system |
|
| |
method |
2-step polymer conjugate |
| |
producer, product no. |
Biocare Medical, NS |
| |
incubation time/temp. |
30 min./RT |
| Chromogen |
|
| |
type |
DAB |
| |
producer, product no. |
Biocare Medical |
| |
incubation time/temp. |
5 min./RT |
| |
enhancement, type |
NS |
| Immunostainer |
|
| |
type |
IntelliPath/ Biocare Medical |
| |
|
|
| Abbreviations |
|
| |
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature |
|
Disclaimer |
|
| |
NordiQC makes every
attempt to provide accurate and up-to-date
information, yet NordiQC does not make any claim or
warranty regarding the accuracy of the provided
information nor does it represent that the contents of
the web site and protocols reflect the most recent
developments in immunohistochemistry at any point in
time. |
|
