|
Protocol provided by |
|
| |
laboratory |
Klinisk patologi/cytologi Huddinge |
| |
hospital/institution |
Karolinska universitetessjukhuset,
Stockholm, Sweden |
| |
e-mail address |
sairam.omar-jossef@karolinska.se |
|
Primary antibody |
|
|
|
clone |
SP4 |
|
|
producer |
NeoMarkers |
|
|
product no., lot no. |
9104-S0, 9104S1008L |
|
|
dilution |
1:50 |
|
|
diluent buffer and additive(s) |
Antibody diluent, Bond |
|
|
incubation time/temp. |
30 min./RT |
| Epitope retrieval, HIER |
|
| |
device |
Bond-max™ |
| |
buffer type, pH |
Bond Epitope Retrieval Sol. 2, pH 9 |
| |
warm-up/heating max/resting time |
NS/20 min./NS |
| |
maximum heating temp. |
100ºC |
| Visualization system |
|
| |
method |
3-step polymer conjugate |
| |
producer, product no. |
Leica Microsystems, DS9800 |
| |
incubation time/temp. |
8 min. + 8 min./RT |
| Chromogen |
|
| |
type |
DAB |
| |
producer, product no. |
Leica Microsystems, DS9800 |
| |
incubation time/temp. |
5 min./RT |
| |
enhancement, type |
Bond DAB Enhancer |
| Immunostainer |
|
| |
type |
Bond-max™, Leica Microsystems |
| |
|
|
| Abbreviations |
|
| |
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature |
|
Disclaimer |
|
| |
NordiQC makes every
attempt to provide accurate and up-to-date
information, yet NordiQC does not make any claim or
warranty regarding the accuracy of the provided
information nor does it represent that the contents of
the web site and protocols reflect the most recent
developments in immunohistochemistry at any point in
time. |
|
