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Protocol provided by |
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laboratory |
Afdeling for klinisk patologi |
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hospital/institution |
Svendborg Sygehus, Svendborg, Denmark |
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e-mail address |
miha@shf.fyns-amt.dk |
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Primary antibody |
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clone |
WT49 |
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producer |
Novocastra |
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product no., lot no. |
NCL-L-WT1-562, L156218 |
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dilution |
1:25 |
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|
diluent buffer and additive(s) |
1 % BSA in TBS |
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|
incubation time/temp. |
30 min./RT |
| Epitope retrieval, HIER |
|
| |
device |
Microwave oven |
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buffer type, pH |
Tris-EDTA/EGTA, pH 9 |
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warm-up/heating max/resting time |
10 min./15 min./15 min. |
| |
maximum heating temp. |
100ºC |
| Visualization system |
|
| |
method |
Polymer conjugate |
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producer, product no. |
Dako, K4007 |
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incubation time/temp. |
30 min./RT |
| Chromogen |
|
| |
type |
DAB |
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producer, product no. |
Dako, K4007 |
| |
incubation time/temp. |
10 min./RT |
| |
enhancement, type |
0.5% cobber sulphate |
| Immunostainer |
|
| |
type |
Autostainer, Lab Vision |
| |
|
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| Abbreviations |
|
| |
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature |
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Disclaimer |
|
| |
NordiQC makes every
attempt to provide accurate and up-to-date
information, yet NordiQC does not make any claim or
warranty regarding the accuracy of the provided
information nor does it represent that the contents of
the web site and protocols reflect the most recent
developments in immunohistochemistry at any point in
time. |
|
