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Protocol provided by |
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laboratory |
Klinik for Patologisk Anatomi |
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hospital/institution |
Sydvestjysk Sygehus Esbjerg, Denmark |
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e-mail address |
IHC@ribeamt.dk |
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Primary antibody |
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clone |
13H4 + 4A4 + D5/16/B4 |
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producer |
Dako |
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product no., lot no. |
M3616 / M7247 / M7237;
08500B / 00025982 / 0051035 |
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dilution |
1:800 / 1:200 / 1:200 |
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|
diluent buffer and additive(s) |
Ab Diluent K8006, Dako |
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|
incubation time/temp. |
20 min./RT |
| Epitope retrieval, HIER |
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| |
device |
PT Link, Dako |
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buffer type, pH |
TRS High pH, Dako K8002 |
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warm-up/heating max/resting time |
15 min./20 min./35 min. |
| |
maximum heating temp. |
97ºC |
| Visualization system |
|
| |
method |
Polymer conjugate |
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producer, product no. |
Dako, K8002 |
| |
incubation time/temp. |
20 min./RT |
| Chromogen |
|
| |
type |
DAB+ |
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producer, product no. |
Dako, K8002 |
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incubation time/temp. |
2x5 min./RT |
| |
enhancement, type |
0,5% Cuppersulfate |
| Immunostainer |
|
| |
type |
Autostainer Link 48, Dako |
| |
|
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| Abbreviations |
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| |
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature |
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Disclaimer |
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| |
NordiQC makes every
attempt to provide accurate and up-to-date
information, yet NordiQC does not make any claim or
warranty regarding the accuracy of the provided
information nor does it represent that the contents of
the web site and protocols reflect the most recent
developments in immunohistochemistry at any point in
time. |
|
