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Protocol provided by |
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laboratory |
Immunlab. Patologisk Inst. |
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hospital/institution |
Århus Sygehus, Denmark |
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e-mail address |
tnord@as.aaa.dk |
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Primary antibody |
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|
clone |
Polyclonal |
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producer |
DakoCytomation |
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product no., lot no. |
A4502, 1J020A |
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dilution |
1:200 |
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|
diluent buffer and additive(s) |
Ab diluent 251-018, Ventana |
|
|
incubation time/temp. |
32 min/NS |
| Epitope retrieval, HIER |
|
| |
device |
Heatingblock, BenchmarkXT |
| |
buffer type, pH |
CC1 (Tris-EDTA/EGTA) |
| |
warm-up/heating max/resting time |
30 min. |
| |
maximum heating temp. |
95ºC |
| Visualization system |
|
| |
method |
Biotin-streptavidin system |
| |
producer, product no. |
Ventana iView, 760-091 |
| |
incubation time/temp. |
16 min./NS |
| Chromogen |
|
| |
type |
DAB |
| |
producer, product no. |
Ventana iView 760-091 |
| |
incubation time/temp. |
4 min./NS |
| |
enhancement, type |
Copper |
| Immunostainer |
|
| |
type |
Benchmark XT, Ventana |
| |
|
|
| Comments |
|
| |
+ Endogenous biotin blocking |
| Abbreviations |
|
| |
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature |
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Disclaimer |
|
| |
NordiQC makes every
attempt to provide accurate and up-to-date
information, yet NordiQC does not make any claim or
warranty regarding the accuracy of the provided
information nor does it represent that the contents of
the web site and protocols reflect the most recent
developments in immunohistochemistry at any point in
time. |
|
