|
Protocol provided by |
|
| |
laboratory |
Dipartiment di Patologia umana ed
oncologia |
| |
hospital/institution |
Careggi, Firenze, Italy |
| |
e-mail address |
milena paglierani@katamail.com |
|
Primary antibody |
|
|
|
clone |
Ber-H2 |
|
|
producer |
Ventana |
|
|
product no., lot no. |
790-2926, 432590 |
|
|
dilution |
ready to use |
|
|
diluent buffer and additive(s) |
NS |
|
|
incubation time/temp. |
32 min./NS |
| Epitope retrieval, HIER |
|
| |
device |
CC1 standard in BenchMark Ventana
Immunostainer |
| |
buffer type, pH |
CC1 (Ethylendiaminetetraacetic acid,
Tris Base, Bori Acid) |
| |
max. temp./max. temp. time |
98ºC/60
min. |
| |
total heating time/resting time |
60 min./8 min. |
| Visualization system |
|
| |
method |
Lab. streptavidin-biotin |
| |
producer, product no. |
Ventana, iVIEW DAB Dection kit, cat.
no. 760-091 |
| |
incubation time/temp. |
8 + 8 min./NS |
| Chromogen |
|
| |
type |
DAB |
| |
producer, product no. |
Ventana, iVIEW DAB Dection kit, cat.
no. 760-091 |
| |
incubation time/temp. |
8 min./NS |
| |
enhancement, type |
iVIEW Copper, iVIEW Detection kit |
| Immunostainer |
|
| |
type |
BenchMark XT Ventana |
| |
|
|
| Abbreviations |
|
| |
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature |
|
Disclaimer |
|
| |
NordiQC makes every
attempt to provide accurate and up-to-date
information, yet NordiQC does not make any claim or
warranty regarding the accuracy of the provided
information nor does it represent that the contents of
the web site and protocols reflect the most recent
developments in immunohistochemistry at any point in
time. |
|
