alternative to CD68, the participating
laboratories could use CD14, which should be performed on the same
material as for CD68.
slide to be stained for
Liver, 3. Juvenile xanthogranuloma, 4.
Myeloid leukaemia FAB M4,
Criteria for assessing a
staining as optimal included: A strong and distinct cytoplasmic and membranous staining of
follicular dendritic cells (in the appendiceal germinal centres),
hepatic Kupffer cells and macrophages in, e.g., the appendix, the meningioma
juvenile xanthogranuloma as well as the myeloid leukaemia,
whereas other cells, such as enterocytes and the
neoplastic cells of the meningioma should
7 laboratories submitted stainings. At the
assessment 6 achieved optimal staining (86 %) and 1 good (14 %).
None were assessed as borderline or poor.
The following mAb was used:
clone 7 (Novocastra; n=7)
In the optimal protocols all used HIER (MWO,
n=5; pressure cooker, n=1) with Tris-EDTA/EGTA pH 9 as the heating
buffer. Clone 7 was used in the range of 1:50-150 depending on the
total sensitivity of the protocols used.
In all protocols the the myeloid leukaemia and
the juvenile xanthogranuloma were appropriately demonstrated. The
reaction pattern was almost identical to that of
CD68 (clones PG-M1) throughout all the stained specimens except for
staining the follicular dendritic cells and hepatic