|
Protocol provided by |
|
| |
laboratory |
Pathology Laboratory |
| |
hospital/institution |
Tampere University Hospital, Finland |
| |
e-mail address |
immo.rantala@pshp.fi |
|
Primary antibody |
|
|
|
clone |
E29 |
|
|
producer |
DakoCytomation |
|
|
product no., lot no. |
M0613, 072(101) |
|
|
dilution |
1:500 |
|
|
diluent buffer and additive(s) |
DakoCytomation antibody diluent S2022 |
|
|
incubation time/temp. |
25 min./RT |
| Epitope retrieval, HIER |
|
| |
device |
MWO |
| |
buffer type, pH |
Tris-EDTA/EGTA, pH 9 |
| |
max. temp./max. temp. time |
99,0ºC/9
min. |
| |
total heating time/resting time |
2 x 7 min./20 min. |
| Visualization system |
|
| |
method |
Polymer conjugate, EnVision |
| |
producer, product no. |
DakoCytomation, K5007 |
| |
incubation time/temp. |
25 min./RT |
| Chromogen |
|
| |
type |
DAB |
| |
producer, product no. |
DakoCytomation, K5007 |
| |
incubation time/temp. |
3 x 5 min./RT |
| |
enhancement, type |
0.5 % CuSO4 in 50mM TBS pH
7.4 ( 5 min./RT ) |
| Immunostainer |
|
| |
type |
TechMate 500 Plus |
| |
|
|
| Abbreviations |
|
| |
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature |
|
Disclaimer |
|
| |
NordiQC makes every
attempt to provide accurate and up-to-date
information, yet NordiQC does not make any claim or
warranty regarding the accuracy of the provided
information nor does it represent that the contents of
the web site and protocols reflect the most recent
developments in immunohistochemistry at any point in
time. |
|
