|
Nine laboratories participated. In one
Hodgkin's lymphoma, 4 labs. got a good result, with an intense
staining of Hodgkin's cells, 3 an acceptable result, and 2 a
poor result. However, in another Hodgkin's lymphoma, only 3
labs. got a good result, while the others a poor result. A
relatively high concentration of antibody and an alkaline buffer
for heat induced epitope retrieval seem to be of major importance
for a good result. Other factors in question are the
incubation temperature, visualization kit and chromogen. The
staining results with two good and two poor protocols are
illustrated below, and clues to the differences in protocols are
indicated.
Good
protocol 1 Good
protocol 2 |
|
Lab. A. CD15 staining of a case of Hodgkin's
lymphoma (MC) using from a good
protocol (1). The Hodgkin's cells as well as the neutrophils are
strongly stained |
Lab. B. CD15 staining of another case of Hodgkin's
lymphoma (MC) from a good
protocol (2) showing the same staining results as in Lab A. |
|
Lab. C. CD15 staining from a poor protocol. Arrows indicate
Hodgkin's cells with a faint Golgi-staining or unstained.
Neutrophilic granulocytes are strongly stained giving a false
impression of a good staining. |
Lab. D. CD15 staining from another poor protocol. Arrows indicate
the virtually unstained Hodgkin's cells.
|
