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Good Protocol no
1 - Run 2 2000
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p53
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Epitope |
P53 |
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Antibody, clone |
DO7 |
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Antibody, producer |
Novocastra |
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Antibody, product no. |
NCL-p53-DO7 |
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Antibody, dilution |
1:40. |
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Antibody, diluent buffer |
DAKO Antibody diluent S2022 |
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Antibody, detergent type |
?
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Antibody, additive |
?
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Antibody, incub. time |
25 min. |
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Antibody, incub. temp. |
Room |
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Heat induced epitope retrieval |
MWO |
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HIER, buffer type |
Citrate pH 6 |
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HIER, buffer molarity |
10 mM |
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HIER, max. temp. |
100 C |
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HIER, total time |
14 min. |
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HIER, max. temp. time |
10 min. |
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HIER, resting time |
20 min. |
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Visualization system |
LSAB |
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Visualization, producer |
DAKO |
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Visualization, product no |
Chemmate K5001 |
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Visualization, diluent buffer |
Ready-to-use |
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Visualization, incub. time |
25 min. |
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Visualization, temp. |
Room |
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Chromogen, type |
DAB |
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Chromogen, producer |
DAKO |
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Chromogen, code |
K5001 |
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Chromogen, incub. time |
15 min. |
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Chromogen, temp. |
Room |
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Chromogen, enhancem. |
?
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Control tissue |
?
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Immunostainer |
TechMate 500+ |
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Laboratory |
Institute of Pathology |
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Hospital |
Tampere University Hospital |
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Address |
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City |
Tampere
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Postal code |
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Country |
Finland
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Technician, name |
Riita Koivisto |
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Technician, e-mail |
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Pathologist, name |
Heikki Helin |
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Pathologist, e-mail |
(se home-page)
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