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Good Protocol no
2 - Run 5 2002
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Ki-67 |
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Epitope |
Ki-67 |
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Antibody, clone |
MIB-1 |
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Antibody, producer |
Immunotech |
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Antibody, product no. |
505 |
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Antibody, dilution |
1:200 |
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Antibody, diluent buffer |
TBS pH 7.6 |
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Antibody, detergent type |
None |
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Antibody, additive |
BSA 1% |
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Antibody, incub. time |
30 min. |
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Antibody, incub. temp. |
Room |
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Heat induced epitope retrieval |
MWO |
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HIER, buffer type |
Tris/EGTA pH 9 |
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HIER, buffer molarity |
10/1 mMol
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HIER, max. temp. |
100ºC |
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HIER, total time |
18 min. |
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HIER, max. temp. time |
15 min. |
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HIER, resting time |
15 min. |
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Visualization system |
Polymer Conjugate |
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Visualization, producer |
DAKO |
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Visualization, product
name and no |
K4007, Envision+
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Visualization, diluent buffer |
Ready-To-Use |
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Visualization, incub. time |
30 min. |
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Visualization, temp. |
Room |
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Chromogen, type |
DAB+ |
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Chromogen, producer |
DAKO |
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Chromogen, code |
K4007 |
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Chromogen, conc. |
1:50 (Kit) |
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Chromogen, incub. time |
? |
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Chromogen, temp. |
Room |
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Chromogen, enhancem. |
None |
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Immunostainer |
DAKO Autostainer |
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Laboratory |
Dept. of Pathology |
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Hospital |
Herlev Hospital |
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Country |
Denmark |
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Contact person |
Anette Enghøj |
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E-mail |
anes@herlevhosp.kbh.amt.dk |
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