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Good Protocol no
1 - Run 5 2002
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Ki-67 |
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Epitope |
Ki-67 |
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Antibody, clone |
MIB-1 |
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Antibody, producer |
DAKO |
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Antibody, product no. |
M7240 |
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Antibody, dilution |
1:100 |
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Antibody, diluent buffer |
DAKO S2022 |
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Antibody, detergent type |
None |
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Antibody, additive |
None |
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Antibody, incub. time |
40 min. |
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Antibody, incub. temp. |
Room |
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Heat induced epitope retrieval |
Pressure Cooker
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HIER, buffer type |
Citrate pH 6.0 |
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HIER, buffer molarity |
100 mMol
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HIER, max. temp. |
120ºC |
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HIER, total time |
10 min. |
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HIER, max. temp. time |
5 min. |
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HIER, resting time |
20 min. |
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Visualization system |
LSAB |
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Visualization, producer |
DAKO |
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Visualization, product
name and no |
ChemMate, K5001 |
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Visualization, diluent buffer |
Ready-To-Use |
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Visualization, incub. time |
25 min. |
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Visualization, temp. |
Room |
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Chromogen, type |
DAB |
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Chromogen, producer |
DAKO |
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Chromogen, code |
K5001 |
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Chromogen, conc. |
RTU |
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Chromogen, incub. time |
? |
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Chromogen, temp. |
Room |
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Chromogen, enhancem. |
None |
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Immunostainer |
DAKO TechMate 500+ |
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Laboratory |
Dept. of Pathology |
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Hospital |
St. Olav's Hospital,
Trondheim |
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Country |
Norway |
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Contact person |
Sverre Torp |
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E-mail |
sverre.torp@rit.no |
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