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Run 6/2002 Good Protocol
2 |
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Calretinin |
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Origin |
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laboratory |
Dept. of Pathology, Skive, Denmark |
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e-mail address |
sspatbkm@vibamt.dk |
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Antibody |
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clone |
DAK calret1 |
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producer, product no. |
DakoCytomation, M7245 |
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dilution/buffer, additive |
1:100/NS |
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inubation time/temp. |
25
min./RT |
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Epitope retrieval |
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type |
HIER in MWO |
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buffer type, molarity |
10/0,5 mM Tris/EGTA pH 9 |
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max. temp./max. temp. time |
100ºC/10
min. |
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total heating time/resting time |
14
min./20 min. |
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Visualization system |
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method |
Chemmate Envision |
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producer, product no. |
DakoCytomation, K5007 |
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incubation time/temp. |
25
min./RT |
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Chromogen |
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type |
DAB+ |
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producer, product no. |
DakoCytomation |
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incubation time/temp. |
15
min./RT |
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enhancement |
none |
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Immunostainer |
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type |
DakoCytomation Horizon |
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Abbreviations:
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature
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SN/MV |
Last
update
20-08-2003 |
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