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Run 6/2002 Good Protocol 1 |
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Calretinin |
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Origin |
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laboratory |
Dept. of Pathology, Ålesund, Norway |
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e-mail address |
ole.steffensen@smr.no,
bjoern.westre@smr.no |
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Antibody |
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clone |
5A5 |
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producer, product no. |
Novocastra, NCL-Calretinin |
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dilution/buffer, additive |
1:100/NS |
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inubation time/temp. |
30
min./RT |
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Epitope retrieval |
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type |
HIER in MWO |
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buffer type, molarity |
10/1 mM Tris/EDTA pH 9 |
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max. temp./max. temp. time |
100ºC/13
min. |
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total heating time/resting time |
18
min./15 min. |
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Visualization system |
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method |
Envision+ |
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producer, product no. |
DakoCytomation, K4001 |
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incubation time/temp. |
30
min./RT |
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Chromogen |
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type |
DAB+ |
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producer, product no. |
DakoCytomation, K3468 |
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incubation time/temp. |
5
min./RT |
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enhancement |
None |
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Immunostainer |
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type |
DakoCytomation Autostainer |
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Abbreviations:
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature
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SN/MV |
Last
update
20-08-2003 |
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