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Good Protocol no
2 - Run 5 2002
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Synaptophysin |
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Epitope |
Synaptophysin |
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Antibody, clone |
Snp88 |
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Antibody, producer |
BioGenex |
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Antibody, product no. |
MU363-UC |
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Antibody, dilution |
1:50 |
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Antibody, diluent buffer |
TBS pH 7.6 |
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Antibody, detergent type |
Tween20 0.05%
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Antibody, additive |
Biotin 0.005% |
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Antibody, incub. time |
30 min. |
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Antibody, incub. temp. |
Room |
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Heat induced epitope retrieval |
MWO
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HIER, buffer type |
Tris/EGTA pH
9 |
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HIER, buffer molarity |
10/1 mMol
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HIER, max. temp. |
100ºC |
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HIER, total time |
25 min. |
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HIER, max. temp. time |
10 min. |
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HIER, resting time |
10 min. |
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Visualization system |
Avidin Biotin Complex |
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Visualization, producer |
Vector |
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Visualization, product
name and no |
Elite, PK7200 |
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Visualization, diluent buffer |
Ready-To-Use |
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Visualization, incub. time |
30 min. |
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Visualization, temp. |
Room |
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Chromogen, type |
Novared |
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Chromogen, producer |
Vector |
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Chromogen, code |
SK4800 |
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Chromogen, conc. |
Kit |
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Chromogen, incub. time |
? |
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Chromogen, temp. |
Room |
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Chromogen, enhancem. |
none |
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Immunostainer |
Manual |
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Laboratory |
Institute of Pathology |
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Hospital |
Næstved
Hospital |
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Country |
Denmark |
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Contact person |
Michael Bzorek |
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E-mail |
mbz@cn.stam.dk
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