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Good Protocol no
2 - Run 2 2000
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Cytokeratin, low molecular weight
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Epitope |
Cytokeratin 8 (or CK18?) |
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Antibody, clone |
C51 |
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Antibody, producer |
Zymed |
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Antibody, product no. |
18-185 |
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Antibody, dilution |
1:100 |
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Antibody, diluent buffer |
0,05 M TBS pH 7,4 |
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Antibody, detergent type |
0,05 % Tween 20 |
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Antibody, additive |
1 % BSA / 150 mM NaCl |
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Antibody, incub. time |
30 min. |
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Antibody, incub. temp. |
Room |
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Heat induced epitope retrieval |
MWO |
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HIER, buffer type |
Tris/EDTA pH 9 |
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HIER, buffer molarity |
10/1 mM |
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HIER, max. temp. |
100ºC |
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HIER, total time |
22 min. |
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HIER, max. temp. time |
15 min. |
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HIER, resting time |
15 min. |
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Biotin blocking |
No |
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Visualization system |
Polymer conjugate |
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Visualization, producer |
DAKO |
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Visualization, product no |
Envision+ K4001 |
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Visualization, diluent buffer |
Ready-to-use |
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Visualization, incub. time |
30 min. |
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Visualization, temp. |
Room |
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Chromogen, type |
DAB+ |
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Chromogen, producer |
DAKO |
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Chromogen, code |
K3468 |
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Chromogen, conc. |
1:50. |
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Chromogen, incub. time |
10 min. |
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Chromogen, temp. |
Room |
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Chromogen, enhancem. |
0,5 % CuSO4 for 5 min. |
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Control tissue |
Colon, tonsil and liver |
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Immunostainer |
Autostainer |
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Laboratory |
Institute of Pathology |
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Hospital |
Aalborg |
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Address |
Box 561 |
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City |
Aalborg |
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Postal code |
DK-9100 |
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Country |
Denmark |
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Technician, name |
Søren Nielsen |
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Technician, e-mail |
u13021@aas.nja.dk |
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Pathologist, name |
Mogens Vyberg |
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Pathologist, e-mail |
vyberg@aas.nja.dk |
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