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Run 6/2002 Good Protocol
2 |
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Epithelial antigen
Ber-EP4 |
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Origin |
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laboratory |
Dept. of
Pathology, Rigshospitalet, Denmark |
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e-mail
address |
rh12904@rh.dk |
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Antibody |
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clone |
Ber-EP4 |
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producer,
product no. |
DakoCytomation, M0804 |
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dilution/buffer,
additive |
1:20/NS |
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inubation
time/temp. |
60 min./RT |
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Epitope
retrieval |
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type |
Proteolytic
pretreatment |
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enzyme |
Proteinase
K |
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producer,
product no. |
DakoCytomation, S3020 |
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method |
5 min. at
RT |
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Visualization system |
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method |
Chemmate
Envision |
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producer,
product no. |
DakoCytomation, K5007 |
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incubation
time/temp. |
25 min./RT |
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Chromogen |
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type |
DAB |
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producer,
product no. |
DakoCytomation, K5007 |
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incubation
time/temp. |
15 min./RT |
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enhancement |
None |
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Immunostainer |
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type |
Dako Cytomation Techmate 500+ |
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Abbreviations:
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature
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SN/MV |
Last
update
20-08-2003 |
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