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Run 7/2003 Good Protocol
3 |
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CD117
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Protocol provided by |
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laboratory |
Dept. of Pathology |
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hospital/institution |
Vestfold Hospital, Tønsberg, Norway |
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e-mail address |
marianne.ludahl@siv.no |
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Primary antibody |
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clone |
polyclonal |
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producer |
DakoCytomation |
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product no., lot no. |
A4502, 2E023A |
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dilution |
1:200 |
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diluent buffer and additive(s) |
antibody diluent, Ventana,
215-018 |
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incubation time/temp. |
30 min./
41°C |
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Epitope retrieval, HIER |
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device |
warm plate in the BenchMark |
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buffer type, pH |
Tris-EDTA/EGTA, pH 9 |
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max. temp./max. temp. time |
100°C/ 20 min. |
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total heating time/resting time |
28 min. |
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Visualization system |
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method |
avidin-biotin-complex |
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producer, product no. |
Ventana, 760-091 |
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incubation time/temp. |
32 min./41°C |
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Chromogen |
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type |
DAB |
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producer, product no. |
iView DAB, Ventana, 253-2190 |
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incubation time/temp. |
8 min./41°C |
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enhancement, type |
copper |
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Immunostainer |
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type |
BenchMark, Ventana |
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Comments |
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Amplifier: Rabbit anti-Mouse and Mouse anti-Rabbit (IgG
heavy and light chains) between
first and second antibody. |
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Abbreviations |
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HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature |
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Disclaimer |
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NordiQC makes every
attempt to provide accurate and up-to-date
information, yet NordiQC does not make any claim or
warranty regarding the accuracy of the provided
information nor does it represent that the contents of
the web site and protocols reflect the most recent
developments in immunohistochemistry at any point in
time. |
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SN/AS |
Last
update
20-08-2003 |
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