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Run 6/2002 Good Protocol 2 |
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CD79a |
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Origin |
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laboratory |
Dept. of
Pathology, Karolinska Sgh., Sweden |
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e-mail
address |
marianne.lagnefeldt@ks.se,
goran.elmberger@ks.se |
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Antibody |
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clone |
JCB117 |
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producer,
product no. |
DakoCytomation, M7050 |
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dilution/buffer,
additive |
1:100/NS |
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inubation
time/temp. |
32
min./37ºC. |
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Epitope
retrieval |
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type |
HIER in MWO |
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buffer type, molarity |
Citrate pH
7,3 |
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max.
temp./max. temp. time |
100ºC/NS |
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total
heating time/resting time |
20 min.20
min. |
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Visualization system |
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method |
Avidin-biotin-complex |
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producer,
product no. |
Ventana Enhanced DAB kit 760-003 |
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incubation
time/temp. |
8 min./37ºC |
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Chromogen |
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type |
DAB |
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producer,
product no. |
Ventana,
760-003 |
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incubation
time/temp. |
8 min./37ºC |
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enhancement |
Copper |
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Immunostainer |
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type |
Ventana Nexes |
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Abbreviations:
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature
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SN/MV |
Last
update
20-08-2003 |
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