|
|
|
|
Run 6/2002 Good Protocol 1 |
|
CD79a |
|
Origin |
|
|
|
laboratory |
Dept. of Pathology, Vejle, Denmark |
|
|
e-mail address |
janlin@vs.vejleamt.dk |
|
Antibody |
|
|
|
clone |
JCB117 |
|
|
producer, product no. |
DakoCytomation, M7050 |
|
|
dilution/buffer, additive |
1:100/NS |
|
|
inubation time/temp. |
30 min./RT |
|
Epitope retrieval |
|
|
|
type |
HIER in MWO |
|
|
buffer type, molarity |
10/0,5 mM Tris/EGTA pH 9 |
|
|
max. temp./max. temp. time |
100ºC/15 min. |
|
|
total heating time/resting time |
26 min./15 min. |
|
Visualization system |
|
|
|
method |
Envision+ |
|
|
producer, product no. |
DakoCytomation K4007 |
|
|
incubation time/temp. |
30 min./RT |
|
Chromogen |
|
|
|
type |
DAB |
|
|
producer, product no. |
DakoCytomation K4007 |
|
|
incubation time/temp. |
10 min./RT |
|
|
enhancement |
None |
|
Immunostainer |
|
|
|
type |
Labvision Autostainer |
|
|
Abbreviations:
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature
|
SN/MV |
Last
update
20-08-2003 |
|
|
|
|