Epitope

CD5

Antibody, clone

4C7

Antibody, product no.

NCL-CD5-4C7

Antibody, producer

Novocastra

Antibody, dilution

1:40

Antibody, diluent buffer

TBS

Antibody, detergent type

?

Antibody, additive

2 % BSA

Antibody, incubation time

30 min.

Antibody, incubationtemp.

Room

Heat induced epitope retrieval (HIER)

MWO

HIER, buffer type

EDTA pH 8

HIER, buffer molarity

1 mM

HIER, max. temp.

100

HIER, total time

20 min.

HIER, max. temp. time

15 min.

HIER, resting time

15 min.

Visualization system

Polymer conjugate

Visualization, producer

DAKO

Visualization, name and product no

EnVision+ K4007

Visualization, diluent buffer

Ready-to-use

Visualization, incub. time

30 min.

Visualization, temp.

Room

Chromogen, type

DAB

Chromogen, producer

DAKO

Chromogen, code

K4007

Chromogen, conc.

1:50.

Chromogen, enhancem.

Copper

Control tissue

Tonsil

Immunostainer

Manual

Laboratory

Dept.of Pathology

Hospital

The Norwegian Radium Hospital

Address

City

Oslo

Postal code

N-

Country

Norway

Technician, name

Grethe Mykkelbost

Technician, e-mail

Pathologist, name

Emina Torlakovic

Pathologist, e-mail