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Good Protocol no
2 - Run 5 2002
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CD3 |
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Epitope |
CD3 |
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Antibody, clone |
Polyclonal |
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Antibody, producer |
DAKO |
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Antibody, product no. |
A0452 |
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Antibody, dilution |
1:400 |
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Antibody, diluent buffer |
DAKO S 2022 |
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Antibody, detergent type |
Triton-X 100 |
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Antibody, additive |
None |
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Antibody, incub. time |
30 min. |
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Antibody, incub. temp. |
Room |
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Heat induced epitope retrieval |
Pressure Cooker
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HIER, buffer type |
Tris/EDTA pH 9.0 |
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HIER, buffer molarity |
10/1 mMol
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HIER, max. temp. |
100ºC ? |
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HIER, total time |
6 min.
? |
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HIER, max. temp. time |
6 min.
? |
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HIER, resting time |
20 min. |
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Visualization system |
Polymer Conjugate |
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Visualization, producer |
DAKO |
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Visualization, product
name and no |
K5007, EnVision
ChemMate |
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Visualization, diluent buffer |
Ready-To-Use |
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Visualization, incub. time |
30 min. |
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Visualization, temp. |
Room |
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Chromogen, type |
DAB+ |
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Chromogen, producer |
DAKO |
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Chromogen, code |
K5007 |
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Chromogen, conc. |
1:50 (kit) |
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Chromogen, incub. time |
? |
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Chromogen, temp. |
Room |
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Chromogen, enhancem. |
None |
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Immunostainer |
DAKO TechMate 500 |
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Laboratory |
Institute of Pathology |
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Hospital |
Linköping University
Hospital |
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Country |
Sweden |
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Contact person |
Birgitta Fränlund |
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E-mail |
birgitta.franlund@lio.se |
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