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Run 6/2002 Good Protocol
2 |
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CD20 |
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Origin |
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laboratory |
Dept. of Pathology, Skive, Denmark |
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e-mail address |
sspatbkm@vibamt.dk, sspatgps@vibamt.dk |
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Antibody |
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clone |
L26 |
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producer, product no. |
DakoCytomation, M0755 |
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dilution/buffer,additive |
1:300/NS |
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inubation time/temp. |
25 min./RT |
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Epitope retrieval |
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type |
HIER in MWO |
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buffer type, molarity |
10 mM Citrate pH 6 |
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max. temp./max. temp. time |
98ºC/10 min. |
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total heating time/resting time |
14 min./20 min. |
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Visualization system |
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method |
Labelled streptavidin-biotin |
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producer, product no. |
DakoCytomation K5001 |
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incubation time/temp. |
25 min./RT |
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Chromogen |
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type |
DAB |
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producer, product no. |
DakoCytomation K5001 |
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incubation time/temp. |
15 min./RT |
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enhancement |
None |
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Immunostainer |
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type |
DakoCytomation Horizon |
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Abbreviations:
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature
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SN/MV |
Last
update
20-08-2003 |
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