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Run 6/2002 Good Protocol 1 |
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CD20 |
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Origin |
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laboratory |
Dept. of Pathology, Helsingborg, Sweden |
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e-mail address |
gunilla.chebill@helsingborgslasarett.se |
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Antibody |
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clone |
L26 |
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producer, product no. |
DakoCytomation, M0755 |
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dilution/buffer,additive |
1:50/NS |
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inubation time/temp. |
32 min./37ºC |
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Epitope retrieval |
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type |
HIER in MWO |
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buffer type, molarity |
Citrate , pH 7 |
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max. temp./max. temp. time |
98ºC/NS |
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total heating time/resting time |
20 min./5 min. |
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Visualization system |
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method |
Avidin-biotin-complex |
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producer, product no. |
Ventana (DAB Paraffin 253) |
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incubation time/temp. |
8 min./37ºC |
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Chromogen |
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type |
DAB |
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producer, product no. |
Ventana 253 |
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incubation time/temp. |
12 min./37ºC |
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enhancement |
Copper |
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Immunostainer |
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type |
Ventana Nexes |
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Abbreviations:
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature
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SN/MV |
Last
update
20-08-2003 |
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