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Good Protocol no
2 - Run 3 2001
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CD15
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|
Epitope |
CD15 |
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Antibody, clone |
MMA |
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Antibody, producer |
Becton Dickinson |
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Antibody, product no. |
347420 |
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Antibody, dilution |
1:10 |
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Antibody, diluent buffer |
0,05 M TBS pH 7,4 |
|
Antibody, detergent type |
0,05%
Tween 20 |
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Antibody, additive |
1 % BSA/150 mM NaCl |
|
Antibody, incub. time |
30
min. |
|
Antibody, incub. temp. |
Room |
|
Heat induced epitope retrieval |
MWO |
|
HIER, buffer type |
Tris/EDTA pH 9 |
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HIER, buffer molarity |
10/1 mM |
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HIER, max. temp. |
100 C |
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HIER, total time |
22 min. |
|
HIER, max. temp. time |
15 min. |
|
HIER, resting time |
15 min. |
|
Visualization system |
Polymer conjugate |
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Visualization, producer |
DAKO |
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Visualization, product name and no |
EnVision+ K4001 |
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Visualization, diluent buffer |
RTU |
|
Visualization, incub. time |
30 min. |
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Visualization, temp. |
Room |
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Chromogen, type |
DAB+ |
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Chromogen, producer |
DAKO |
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Chromogen, code |
K3468 |
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Chromogen, conc. |
1:50. |
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Chromogen, incub. time |
10 min. |
|
Chromogen, temp. |
Room |
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Chromogen, enhancem. |
0,5 % CuSO 4 |
|
Control tissue |
Hodgkin's
lymphoma
|
|
Immunostainer |
Autostainer |
|
Laboratory |
Institute of Pathology |
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Hospital |
Aalborg |
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Address |
Box 561 |
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City |
Aalborg |
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Postal code |
DK-9100 |
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Country |
Denmark |
|
Technician, name |
Søren Nielsen |
|
Technician, e-mail |
u13021@aas.nja.dk |
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Pathologist, name |
Mogens Vyberg |
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Pathologist, e-mail |
vyberg@aas.nja.dk |
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