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Good Protocol no
1 - Run 3 2001
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CD15
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Epitope |
CD15 |
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Antibody, clone |
C3D-1 |
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Antibody, producer |
DAKO |
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Antibody, product no. |
M0733 |
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Antibody, dilution |
1:10 |
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Antibody, diluent buffer |
Tris |
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Antibody, detergent type |
?
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Antibody, additive |
?
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Antibody, incub. time |
30 min. |
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Antibody, incub. temp. |
Room |
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Heat induced epitope retrieval |
MWO |
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HIER, buffer type |
EDTA pH 8 |
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HIER, buffer molarity |
1mM |
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HIER, max. temp. |
100 C |
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HIER, total time |
20 min. |
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HIER, max. temp. time |
15 min. |
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HIER, resting time |
15 min. |
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Visualization system |
Polymer conjugate |
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Visualization, producer |
DAKO |
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Visualization, product name and no |
EnVision+ K4007 |
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Visualization, diluent buffer |
Ready-to-use |
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Visualization, incub. time |
30 min. |
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Visualization, temp. |
Room |
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Chromogen, type |
DAB+ |
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Chromogen, producer |
DAKO |
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Chromogen, code |
K4007 |
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Chromogen, conc. |
1:50 |
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Chromogen, buffer |
?
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Chromogen, incub. time |
5 min. |
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Chromogen, temp. |
Room |
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Chromogen, enhancem. |
Copper |
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Control tissue |
?
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Immunostainer |
Manual |
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Laboratory |
Dept. of Pathology |
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Hospital |
The Norwegian Radiumhospital |
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Address |
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City |
Oslo |
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Postal code |
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Country |
Norway |
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Technician, name |
Grethe Mykkelbost |
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Technician, e-mail |
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Pathologist, name |
Emina Torlakovic |
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Pathologist, e-mail |
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