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Good Protocol no
1 - Run 4 2001
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CD117
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Epitope |
CD117 |
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Antibody, clone |
Polyclonal |
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Antibody, producer |
DAKO |
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Antibody, product no. |
A4502 |
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Antibody, dilution |
1:200 |
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Antibody, diluent buffer |
DAKO Antibody diluent S2022 |
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Antibody, detergent type |
?
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Antibody, additive |
?
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Antibody, incub. time |
40 min. |
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Antibody, incub. temp. |
Room
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Heat induced epitope retrieval |
Pressure cooker |
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HIER, buffer type |
TRS pH 9,9 DAKO S3307 |
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HIER, buffer molarity |
?
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HIER, max. temp. |
100 ºC |
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HIER, total time |
9 min. |
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HIER, max. temp. time |
5 min. |
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HIER, resting time |
20 min. |
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Visualization system |
LSAB |
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Visualization, producer |
DAKO |
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Visualization, product name and no |
ChemMate K5003 |
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Visualization, diluent buffer |
Ready-to-use |
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Visualization, incub. time |
25 min. |
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Visualization, temp. |
Room |
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Chromogen, type |
AEC |
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Chromogen, producer |
DAKO |
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Chromogen, code |
K5003 |
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Chromogen, conc. |
RTU
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Chromogen, incub. time |
15 min. |
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Chromogen, temp. |
Room |
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Chromogen, enhancem. |
?
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Control tissue |
Colon
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Immunostainer |
DAKO Technate 500+ |
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Laboratory |
Dept. of Pathology |
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Hospital |
Trondheim |
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Address |
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City |
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Postal code |
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Country |
Norway |
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Technician, name |
Ingunn Nervik |
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Technician, e-mail |
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Pathologist, name |
Sverre H Torp |
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Pathologist, e-mail |
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