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Run 6/2002 Good Protocol 1 |
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CD10 |
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Origin |
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laboratory |
Dept. of Pathology, Hillerød Hospital, Denmark |
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e-mail address |
lith@fa.dk, anfala@fa.dk |
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Antibody |
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clone |
56C6 |
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producer, product no. |
Neomarkers, 728S111 |
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dilution/buffer,additive |
1:50/NS |
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inubation time/temp. |
30 min./RT |
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Epitope retrieval |
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type |
HIER in MWO |
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buffer type, molarity |
Tris/EDTA 10/1mM, pH9 |
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max. temp./max. temp. time |
100ºC/15 min. |
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total heating time/resting time |
20 min./15 min. |
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Visualization system |
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method |
ChemMate Envision |
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producer, product no. |
DakoCytomation, K5007 |
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incubation time/temp. |
30 min./RT |
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Chromogen |
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type |
DAB |
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producer, product no. |
DakoCytomation, K5007 |
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incubation time/temp. |
10 min./RT |
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enhancement |
none |
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Immunostainer |
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type |
DakoCytomation Autostainer |
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Abbreviations:
HIER: heat induced epitope retrieval, MWO: microwave oven,
NS: not stated, RT: room temperature
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SN/MV |
Last
update
20-08-2003 |
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