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Current protocols
 

Recommended protocols from NordiQC assessment schemes

Among protocols shown to give optimal staining results, one or more are selected to cover a spectrum of laboratories, antibodies and techniques.

Only the latest recommended protocols for each antibody/clone/epitope are listed here.

All previously recommended protocols are listed in Previous protocols.

A short survey of Abs and protocols from Aalborg and Odense are listed in "Antistoftabel" (pdf-format)

 

mAb = mouse monoclonal antibody, rmAb = rabbit monoclonal antibody, pAb = polyclonal antibody

Epitope

Antibody

Protocols Run 27 and B8  (December 2009)
ASMA mAb 1A4 Næstved, DK  ■  Stockholm, SE  ■  Berlin, DE
CD10 mAb 56C6 Leeuwarden, NL  ■  Amsterdam, NL  ■
Göteborg, Se  ■ Anonymous
CDX2 mAb AMT28 Vilnius, LT
mAb CDX2-88 Aliso Viejo, USA
mAb DAK-CDX2 Halmstad, SE  ■  Aalborg, DK
rmAb EPR2764Y Anonymous
CEA mAb II-7 Helsingborg, SE  ■  Rotterdam, NL  ■
Santiago de Compostela, ES
mAb COL-1 Odense, DK  ■  Drammen, NO
P501s mAb 10E3 Svendborg, DK
PSA mAb 35H9 Mistelbach, AT
mAb ER-PR8 Lillehammer, NO
mAb ER-PR8+PA05 Ålesund, NO
pAb 760-2506 Anonymous
pAb A0562 Århus, DK  ■  Blaricum, NL
pAb IR514 Örebro, SE
ER mAb 1D5 Lahti, FI  ■  Anonymous
mAb 6F11 Rotterdam, NL
rmAb SP1 Gävle, SE
HER-2 IHC rmAb SP3 Drammen, NO
pAb A0485 Barcelona, ES
HercepTest Trollhättan, SE
PATHWAY® Turku, FI
p63 mAb 4A4 Den Haag, NL  ■  Anonymous
mAb 4A4 + Y4A3 Lahti, FI
mAb BC4A4 Bridgewater, CA
SMH mAb S131 Stockholm, SE
mAb SMMS-1 Odense, DK  ■  Anonymous

Epitope

Antibody

All current protocols

Previous protocols

A          B          C          D          E          F          G          H          K          M          P          S          T          V
ALK mAb ALK1 Herlev, DK (Run 17)  ■  Sundsvall, SE (Run 17)
mAb 5A4 Jyväskylä, FI (Run 17)
AMACR mAb 13H4 Anonymous (Run 26)  ■  Hämeenlinna, FI (Run 26)  ■
 Lier, BE (Run 26)  ■  Västerås, SE (Run 26)
pAb CP200C Wien, AT (Run 26)
AMACR/PIN-cocktail cocktail Esbjerg, DK (Run 26)  ■  Kassel, DE (Run 26)  ■
 Slagelse, DK (Run 26)

ASMA

 mAb 1A4 Næstved, DK (Run 27)  ■  Stockholm, SE (Run 27)  ■ Berlin, DE (Run 27)

Bcl-2

mAb 100 

Sønderborg, DK (Run 13) 

mAb 124

Skövde, SE (Run 13)

mAb 8C8

Jyväskylä, FI (Run 13)

mAb bcl-2/100/D5

Stavanger, NO (Run 13)

Bcl-6

 mAb GI191E/A8 Anonymous (Run 17)
mAb PG-B6p Norbyhagen, NO (Run 17)  ■  Seinäjoki, FI (Run 17)

Calretinin (CR)

 mAb 5A5 Rotterdam, NL (Run 23)  ■  Tønsberg, NO (Run 23)
mAb Dak-Calret1 Anonymous (Run 23)  ■ Mistelbach, AT (Run 23)
pAb 18-0211 Stockholm, SE   ■  Wien, AT (Run 23)
CA125 mAb M11 Lund, SE (Run 15)
mAb Ov185:1 Drammen, NO (Run 15)
mAb OC125 Malmö, SE (Run 15)

CD3

 mAb 2GV6 Malmö, SE (Run 22)
mAb F7.2.38 Sarajevo, BA (Run 22)
mAb LN10 Rotterdam, NL (Run 22)

mAb PS1

 Oulu, FI (Run 22)
mAb SP7 Skive, DK (Run 22)

pAb A0452

 Bodø, NO (Run 22)

CD4

mAb 1F6

Luleå, SE (Run 14)  ■  Bergen, NO (Run 14)

mAb 4B12

Odense, DK (Run 14)  ■  Trondheim, NO (Run 14)

CD5

 mAb 4C7 Hämeenlinna, FI (Run 24)  ■  Vejle, DK (Run 24)
rmAb SP19 Drammen, NO (Run 24)  ■  Esbjerg, DK (Run 24)

CD8

mAb 1A5

Irvine, USA (Run 14)

mAb 4B11

Seinäjoki, FI (Run 14)

mAb CD8/144B

Västerås, SE (Run 14)
CD10 mAb 56C6 Leeuwarden, NL (Run 27)  ■  Amsterdam, NL (Run 27)  ■  Göteborg, Se (Run 27)  ■ Anonymous (Run 27)

CD14

mAb 7

Helsinki, FI (Run 11)  ■  Næstved, DK (Run 11)

CD15

mAb C3D-1

 Linköping, SE (Run 25)
mAb Carb-3 Næstved, DK (Run 25)  ■  Barcelona, ES (Run 25)
mAb H198 Kuopio, FI (Run 25)

mAb MMA

 Roskilde, DK (Run 25)  ■  Anonymous (Run 25)

CD20

 mAb L26 Granada, ES (Run 21)  ■  Lahti, FI (Run 21)  ■
 Oslo, NO (Run 21)
pAb RN-9013 Ålesund, NO (Run 21)

CD23

mAb 1B12

 Karolinska, Stockholm, SE (Run 24)  ■
 Stavanger, NO (Run 24)

rmAb SP23

 Haarlem, NL (Run 24)  ■
 Santiago de Compostela, ES (Run 24)

CD30

mAb Ber-H2

 Aliso Viejo, USA (Run 25)
mAb 15B3 Helsingborgs, SE (Run 25)
mAb BER-H2 Borås, SE (Run 25)  ■  Mikkeli, FI (Run 25)

CD31

 mAb JC70a Oslo, NO (Run 26)  ■  Wien, AT (Run 26)  ■
 Ålesund, NO (Run 26)

CD34

mAb My10

RH, København, DK (Run 7)

mAb QBEnd 10

Glostrup, DK (Run 7)  ■  Kristiansand, NO (Run 7)  ■
Næstved, DK (Run 7)

CD45 mAb 2B11+PD7/26 Trondheim, NO (Run 15)  ■  Sundsvall, SE (Run 15)
mAb RP2-18 Irvine, USA (Run 15)
mAb T29/33 Guangzhou, CHN (Run 15)

CD68

 mAb 514H12 Eindhoven, NL (Run 26)

mAb KP1

 Genk, BE (Run 26)  ■  Luleå, SE (Run 26)

mAb PG-M1

 Anonymous (Run 26)  ■  Rotterdam, NL (Run 26)  ■
 Zürich, CH (Run 26)

CD79a

 mAb JCB117 Haag, NL (Run 21)  ■  Roskilde, DK (Run 21)  ■
 Stavanger, NO (Run 21)
rmAb SP18 Bonn, DE (Run 21)  ■  Jyväskylä, FI (Run 21)

CD99

mAb 12E7

Aalborg, DK (Run 12)  ■  Karlstad, SE (Run 12)

CD117

 rmAb YR145 Aalborg, DK (Run 26)  ■  Næstved, DK (Run 26)
pAb A4502 Hillerød, DK (Run 26)  ■  Kassel, DE (Run 26)  ■
 Lahti, FI (Run 26)
CD138 mAb B-A38 Castelfranco Veneto, IT (Run 21)  ■
 Kristiansand, NO (Run 21)
mAb B-B4 Seinajoki, FI (Run 21)
mAb BC/B-B4 Saskatoon, CA (Run 21)
mAb MI15 Linköping, SE (Run 21)
CDX2 mAb AMT-28 Vilnius, LT (Run 27)
mAb CDX2-88 Aliso Viejo, USA (Run 27)
mAb DAK-CDX2 Halmstad, SE (Run 27)  ■  Aalborg, DK (Run 27)
rmAb EPR2764Y Anonymous (Run 27)

CEA

mAb II-7

 Helsingborg, SE (Run 27)  ■  Rotterdam, NL (Run 27)  ■
Santiago de Compostela, ES (Run 27)

mAb COL-1

 Odense, DK  ■  Drammen, NO
Chromogranin (CGA) mAb LK2H10 Treviglio, IT (Run 22)  ■  Uppsala, SE (Run 22)
mAb LK2H10+PHE5 Halmstad, SE (Run 22)
pAb A0430 Gävle, SE (Run 22)  ■  Skien, NO (Run 22)

Cyclin D1

 rmAb SP4 Drammen, NO (Run 19) ■  Rotterdam, NL (Run 19)
mAb P2D11F11 Seinäjoki, FI (Run 19)
Cytokeratin, high molecular weight mAb D5/16 B4 Hillerød, DK (Run B6)
mAb LL002 ME 's-Hertogenbosch, NL (Run B6)
mAb XM26 Aalborg, DK (Run B6)
mAb XM26/LL002 Chemnitz, DE (Run B6)
Cytokeratin, low molecular weight mAb 5D3 Tampere, FI (Run 25)
mAb C51 Kristiansand, NO (Run 25)
mAb CAM 5.2 Skövde, SE (Run 25)
mAb DC10 Guangzhou, CHN (Run 25)  ■  Anonymous (Run 25)

Cytokeratin 5

mAb 34BE12

Irvine, USA (Run 12)  ■  Luleå, SE (Run 12)

mAb D5/16 B4

Seinäjoki, FI (Run 12)  ■  Svendborg, DK (Run 12)

mAb XM26

Kalmar, SE (Run 12)  ■  Stockholm, SE (Run 12)

Cytokeratin 7

mAb OV/TL 12/30

 Seinajoki, FI (Run 25)  ■  Firenze, IT (Run 25)  ■
 Göteborg, SE (Run 25)

Cytokeratin 20

 mAb Ks 20.8 Lillehammer, NO (Run 25)  ■  Malmö, SE (Run 25)  ■
 Esbjerg, DK (Run 25)
pAb E16444 Rotterdam, NL (Run 25)

Cytokeratin, pan-

 mAb AE1/AE3 Chemnitz, DE (Run 24)  ■  Skövde, SE (Run 24)  ■
 Vienna, AT (Run 24)
mAb AE1/AE3+5D3 RH, København, DK (Run 24)
mAb AE1AE3+PCK26 Turku, FI (Run 24)
mAb KL1 Skive, DK (Run 24)
mAb MNF116 Trollhättan, SE (Run 24)

Desmin

 mAb D33 Helsingborg, SE (Run 21)  ■  Wien, AT (Run 21)
mAb DE-R-11 Aliso Viejo, USA (Run 21)  ■  Uppsala, SE (Run 21)
mAb ZN-18 Hämeenlinna, FI (Run 21)
E-cadherin (ECAD) mAb 36B5 Mistelbach, AT (Run B5)
mAb ECH-6 Århus, DK (Run B5)
mAb HECD-1 Göteborg, SE (Run B5)
mAb NCH-38 Haarlem, NL (Run B5)

EMA

mAb E29

Falun, SE (Run 10)  ■  Holstebro, DK (Run 10)  ■
Næstved, DK (Run 10)  ■  Tampere, FI (Run 10)

Epithelial antigen (Ep-CAM)

 mAb Ber-EP4 Norbyhagen, NO (Run 23)  ■  Stockholm, SE (Run 23)
mAb MOC-31 Wien, AT (Run 23)
mAb VU-1D9 Apeldoorn, NL (Run 23)

ER

 mAb 1D5 Lahti, FI (Run B8)  ■  Anonymous (Run B8)
mAb 6F11 Rotterdam, NL (Run B8)
rmAb SP1 Gävle, SE (Run B8)

Factor VIII related antigen

pAb 760-2642

Oslo, NO (Run 11)

pAb A0082

Lindköping, SE (Run 11)  ■  Skövde, SE (Run 11)  ■
Tampere, FI (Run 11)

Glial fibrillary acidic protein

mAb 6F2

Falun, SE (Run 13)  ■  Karlstad, SE (Run 4)

mAb GA5

Kaunas, LT (Run 13)

pAb Z0334

Anonymous (Run 13)  ■  Seinäjoki, FI (Run 13)
Gross cystic disease fluid protein-15 mAb 23A3 Wien, AT (Run 25)  ■  Vilnius, LI (Run 25)
mAb D6 Aliso Viejo, USA (Run 25)  ■  Rotterdam, NL (Run 25)
HER-2 IHC mAb SP3 Drammen, NO (Run B8)
pAb A0485 Barcelona, ES (Run B8)
HercepTest Trollhättan, SE (Run B8)
PATHWAY® Turku, FI (Run B8)

Human chorionic gonadotropin

pAb A231

Esbjerg, DK (Run11)  ■  Holstebro, DK (Run 11)  ■ 
Lund, SE (Run 11)

pAb NCL-HCGp

Firenze, IT (Run 11)
IgK pAb A0191 Jönköping, SE (Run 23)  ■  Odense, DK (Run 23)
IgL pAb A0193 Stockholm, SE (Run 15)  ■  Holstebro, DK (Run 15)
IgM pAb A0425 Bergen, NO (Run 23)  ■  Seinäjoki, FI (Run 23)  ■
Wien, AT (Run 23)
Ki-67 mAb 7B11 Beijing, CHN (Run B7)
mAb MIB1 Bergen, NO (Run B7)  ■  Halmstad, SE (Run B7)
rmAb 30-9 Turku, FI (Run B7)
rmAb SP6 Halle (Saale), DE (Run B7)
Mammaglobin mAb 304-1A5 Vejle, DK (Run 25)  ■  Göteborg, SE (Run 25)
rmAb 31A5 Skövde, SE (Run 25)
MLA mAb A103 Anonymous (Run 24)  ■
 Danderyd, Stockholm, SE (Run 24)  ■
 Odense, DK (Run 24)

Mismatch repair proteins MLH1

mAb G168-15

Hillerød, DK (Run 13)  ■  Irvine, USA (Run 13)  ■
Odense, DK (Run 13)  ■  Trondheim, NO (Run 13)

Mismatch repair proteins MSH2

mAb 25D12

 Trondheim, NO (Run 22)

mAb 27

 Roskilde, DK (Run 22)

mAb FE11

 Næstved, DK (Run 22)

MSA-HMB45

mAb HMB45 (HIER)

Firenze, IT (Run 20)  ■  Lillehammer, NO (Run 20)
mAb HMB45 (None) Berlin, DE (Run 20)  ■  Wien, AT (Run 20)
NFP mAb 2F11 Jönköping, SE (Run 18)  ■  Sundsvall, SE (Run 18)
mAb NFP7, DA2, RmdO20.11 Firenze, IT (Run 18)  ■  Oslo, NO (Run 18)

OCT3/4

 mAb C-10 Rotterdam, NL (Run 24)  ■  Umeå, SE (Run 24)
p16ink4a mAb E6H4 Randers, DK (Run 26)  ■  Stavanger, NO (Run 26)
mAb G175-405 Seinajoki, FI (Run 26)
mAb JC8 Hvidovre, DK (Run 26)
p53 mAb BP53-12 Barcelona, ES (Run 20)
mAb DO-1 Kassel, DE (Run 20)

mAb DO-7

Örebro, SE (Run 20)  ■  Sundsvall, SE (Run 20)
mAb DO-7+BP53-12 Wien, AT (Run 20)
P501s mAb 10E3 Svendborg, DK (Run 27)
p63 mAb 4A4 Den Haag, NL (Run B8)  ■  Anonymous (Run B8)
mAb 4A4 + Y4A3 Lahti, FI (Run B8)
mAb BC4A4 Bridgewater, CA (Run B8)

PAP

mAb PASE/4LJ

Kristiansand, NO (Run 12)  ■  Seinäjoki, FI (Run 12)

pAb A0627

Helsingborg, SE (Run 12)  ■  Holstebro, DK (Run 12)

PLAP

mAb PL8-F6

Næstved, DK (Run 24)
Podop mAb D2-40 Leiden, NL (Run 19)  ■  RH, København, DK (Run 19)
PR mAb 16 Saskatoon, CA (Run B6)
mAb 16+SAN27 Danderyd, Stockholm, SE (Run B6)
mAb 1294 Aliso Viejo, USA (Run B6)
mAb PgR 636 Rijeka, HR (Run B6)
rmAb 1E2 Sundsvall, SE (Run B6)
PSA

mAb 35H9

 Mistelbach, AT (Run 27)

mAb ER-PR8

 Lillehammer, NO (Run 27)

mAb ER-PR8+PA05

 Ålesund, NO (Run 27)

pAb 760-2506

 Anonymous (Run 27)
pAb A0562 Århus, DK (Run 27)  ■  Blaricum, NL (Run 27)

pAb IR514

 Örebro, SE (Run 27)

S-100

pAb Z0311 (Prot)

Luleå, SE (Run 20)  ■  Västerås, SE (Run 20)
pAb Z0311 (HIER) Wien, AT (Run 20)  ■  Århus, DK (Run 20)
SMH mAb S131 Stockholm, SE (Run B8)
mAb SMMS-1 Odense, DK (Run B8)  ■  Anonymous (Run B8)
SYP mAb 27G12 Mikkelin, FI (Run 22)
mAb Snp88 Hillerød, DK (Run 22)
pAb 18-0130 Turku, FI (Run 22)
pAb A0010 Örebro, SE (Run 22)
TdT mAb SEN28 Bergen, NO (Run 18)  ■  Sundsvall, SE (Run 18)
pAb A3524 Herlev, DK (Run 18)  ■  Umeå, SE (Run 18)
pAb 004 Leiden, NL (Run 18)

TTF1

mAb SPT24

 Hämeenlinna, FI (Run 23)  ■  Helsingborg, SE (Run 23)  ■  Trondheim, NO (Run 23)

Vimentin

mAb 3B4

Mikkeli, FI (Run 12)

mAb SP20

Ålesund, NO (Run 12)

mAb V9

Esbjerg, DK (Run 12)  ■  Luleå, SE (Run 12)

WT1

 mAb 6F-H2 Vejle, DK (Run 15)  ■  Helsinki, FI (Run 15)
MV/SN/AS

Last update 03-12-2009