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Current protocols
 

Recommended protocols from NordiQC assessment schemes

Among protocols shown to give optimal staining results, one or more are selected to cover a spectrum of laboratories, antibodies and techniques.

Only the latest recommended protocols for each antibody/clone/epitope are listed here.

All previously recommended protocols are listed in Previous protocols.

A short survey of Abs and protocols from Aalborg and Odense are listed in "Antistoftabel" (pdf-format)

 

mAb = mouse monoclonal antibody, rmAb = rabbit monoclonal antibody, pAb = polyclonal antibody

Epitope

Antibody

Protocols from Run 23 and B5 (July 2008)
Calretinin (CR) mAb 5A5 Rotterdam, NL  ■  Tønsberg, NO
mAb Dak-Calret1 Anonymous  ■  Mistelbach, AT
pAb 18-0211 Stockholm, SE  ■  Wien, AT
Epithelial antigen (Ep-CAM) mAb Ber-EP4 Norbyhagen, NO  ■  Stockholm, SE
mAb MOC-31 Wien, AT
mAb VU-1D9 Apeldoorn, NL
IgK pAb A0191 Jönköping, SE  ■  Odense, DK
IgM pAb A0425 Bergen, NO  ■  Seinäjoki, FI  ■  Wien, AT
TTF1 mAb SPT24 Hämeenlinna, FI  ■  Helsingborg, SE  ■  Trondheim, NO
E-cadherin (ECAD) mAb 36B5 Mistelbach, AT
mAb ECH-6 Århus, DK
mAb HECD-1 Göteborg, SE
mAb NCH-38 Haarlem, NL
Estrogen receptor mAb 1D5 Hjørring, DK
mAb 1D5+ER-2-123 Anonymous
mAb 6F11 Helsinki, FI  ■  Roskilde, DK
rmAb SP1 Roermond, NL  ■  Vejle, DK
HER-2 pAb A0485 Genk, BE
HercepTest Aliso Viejo, USA
Pathway Castelfranco Veneto, IT

Epitope

Antibody

All current protocols

Previous protocols

A          B          C          D          E          F          G          H          K          M          P          S          T          V
ALK mAb ALK1 Herlev, DK (Run 17)  ■  Sundsvall, SE (Run 17)
mAb 5A4 Jyväskylä, FI (Run 17)
AMACR mAb 13H4 Sønderborg, DK (Run 16)  ■  Umeå, SE (Run 16)
pAb ab12498 St.-Petersburg, RU (Run 16)
mAb CP200C Rovaniemi, FI (Run 16)

ASMA

 mAb 1A4 Hillerød, DK (Run 21)  ■  St.-Petersburg, RU (Run 21)

Bcl-2

mAb 100 

Sønderborg, DK (Run 13) 

mAb 124

Skövde, SE (Run 13)

mAb 8C8

Jyväskylä, FI (Run 13)

mAb bcl-2/100/D5

Stavanger, NO (Run 13)

Bcl-6

 mAb GI191E/A8 Anonymous (Run 17)
mAb PG-B6p Norbyhagen, NO (Run 17)  ■  Seinäjoki, FI (Run 17)

Calretinin (CR)

 mAb 5A5 Rotterdam, NL (Run 23)  ■  Tønsberg, NO (Run 23)
mAb Dak-Calret1 Anonymous (Run 23)  ■ Mistelbach, AT (Run 23)
pAb 18-0211 Stockholm, SE   ■  Wien, AT (Run 23)

Carcinoembryonic antigen

mAb II-7

Oslo, NO (Run 12)  ■  Sundsvall, SE (Run 12)

mAb Col-1

Odense, DK (Run 12)
CA125 mAb M11 Lund, SE (Run 15)
mAb Ov185:1 Drammen, NO (Run 15)
mAb OC125 Malmö, SE (Run 15)

CD3

 mAb 2GV6 Malmö, SE (Run 22)
mAb F7.2.38 Sarajevo, BA (Run 22)
mAb LN10 Rotterdam, NL (Run 22)

mAb PS1

 Oulu, FI (Run 22)
mAb SP7 Skive, DK (Run 22)

pAb A0452

 Bodø, NO (Run 22)

CD4

mAb 1F6

Luleå, SE (Run 14)  ■  Bergen, NO (Run 14)

mAb 4B12

Odense, DK (Run 14)  ■  Trondheim, NO (Run 14)

CD5

 mAb 4C7 Aliso Viejo, USA (Run 17)  ■  Lahti, FI (Run 17)
rmAb SP19 Anonymous (Run 17)  ■  Rotterdam, NL (Run 17)

CD8

mAb 1A5

Irvine, USA (Run 14)

mAb 4B11

Seinäjoki, FI (Run 14)

mAb CD8/144B

Västerås, SE (Run 14)
CD10 mAb 56C6 Helsinki, FI (Run 16)  ■ 
ME 's-Hertogenbosch, NL (Run16)  ■  Oslo, NO (Run 16)

CD14

mAb 7

Helsinki, FI (Run 11)  ■  Næstved, DK (Run 11)

CD15

mAb C3D-1

 Rijeka, HR (Run 22)
mAb Carb-3 Gentofte, DK (Run 22)

mAb MMA

 Firenze, IT (Run 22)  ■  Vienna, AT (Run 22)

CD20

 mAb L26 Granada, ES (Run 21)  ■  Lahti, FI (Run 21)  ■
 Oslo, NO (Run 21)
pAb RN-9013 Ålesund, NO (Run 21)

CD23

mAb 1B12

 Randers, DK (Run 19)  ■  Roskilde, DK (Run 19)  ■  Uppsala, SE (Run 19)

rmAb SP23

 Trondheim, NO (Run 19)

CD30

mAb Ber-H2

Borås, SE (Run 11)  ■  Firenze, IT (Run 11)  ■
Jönköping, SE (Run 11)  ■  Odense, DK (Run 11)  ■
 Trondheim, NO (Run 11)

CD31

mAb JC/70A

Odense, DK (Run 11)  ■  Skien, NO (Run 11)  ■
 Sundsvall, SE (Run 11)  ■  Århus, DK (Run 11)

CD34

mAb My10

RH, København, DK (Run 7)

mAb QBEnd 10

Glostrup, DK (Run 7)  ■  Kristiansand, NO (Run 7)  ■
Næstved, DK (Run 7)

CD45 mAb 2B11+PD7/26 Trondheim, NO (Run 15)  ■  Sundsvall, SE (Run 15)
mAb RP2-18 Irvine, USA (Run 15)
mAb T29/33 Guangzhou, CHN (Run 15)

CD68

mAb KP1

Oslo, NO (Run 11)  ■  Växjö, SE (Run 11)

mAb PG-M1

Helsingborg, SE (Run 11)  ■  Stockholm, SE (Run 11)

CD79a

 mAb JCB117 Haag, NL (Run 21)  ■  Roskilde, DK (Run 21)  ■
 Stavanger, NO (Run 21)
rmAb SP18 Bonn, DE (Run 21)  ■  Jyväskylä, FI (Run 21)

CD99

mAb 12E7

Aalborg, DK (Run 12)  ■  Karlstad, SE (Run 12)

CD117

 pAb A4502 Næstved, DK (Run 21)  ■  Rotterdam, NL (Run 21)  ■  Sundsval, SE (Run 21)  ■  Vilnius, LT (Run 21)
CD138 mAb B-A38 Castelfranco Veneto, IT (Run 21)  ■
 Kristiansand, NO (Run 21)
mAb B-B4 Seinajoki, FI (Run 21)
mAb BC/B-B4 Saskatoon, CA (Run 21)
mAb MI15 Linköping, SE (Run 21)
CDX2 mAb AMT-28 Stockholm, SE (Run 22)
mAb CDX2-88 Helsinki, FI (Run 22)
Chromogranin (CGA) mAb LK2H10 Treviglio, IT (Run 22)  ■  Uppsala, SE (Run 22)
mAb LK2H10+PHE5 Halmstad, SE (Run 22)
pAb A0430 Gävle, SE (Run 22)  ■  Skien, NO (Run 22)

Cyclin D1

 rmAb SP4 Drammen, NO (Run 19) ■  Rotterdam, NL (Run 19)
mAb P2D11F11 Seinäjoki, FI (Run 19)
Cytokeratin, high molecular weight mAb 34BE12 Malmö, SE (Run 16)  ■  Skien, NO (Run 16)  ■
 Triviglio, IT (Run 16)
mAb D5/16 B4 Odense, DK (Run 16)
mAb XM26 Karlstad, SE (Run 16)
Cytokeratin, low molecular weight mAb 5D3 Rotterdam, NL (Run 20)
mAb C51 Hjørring, DK (Run 20)
mAb CAM 5.2 Edmonton, CA (Run 20)
mAb DC10 Reykjavik, IS (Run 20)  ■  Treviglio, IT (Run 20)

Cytokeratin 5

mAb 34BE12

Irvine, USA (Run 12)  ■  Luleå, SE (Run 12)

mAb D5/16 B4

Seinäjoki, FI (Run 12)  ■  Svendborg, DK (Run 12)

mAb XM26

Kalmar, SE (Run 12)  ■  Stockholm, SE (Run 12)

Cytokeratin 7

mAb K72

Anonymous (Run 8)

mAb K72.7

Jyväskylä, FI (Run 8)

mAb OV/TL 12/30

Borås, SE (Run 8)  ■  Oslo, NO (Run 8)

Cytokeratin 20

mAb Ks 20.8

Molde, NO (Run 8)  ■  Seinäjoki, FI (Run 8)  ■
 Växjö, SE (Run 8)

Cytokeratin, pan-

 mAb AE1/AE3 Bodø, NO (Run 20)  ■  Karlskrona, SE (Run 20)
mAb AE1/AE3+5D3 Esbjerg, DK (Run 20)
mAb KL1 Bispebjerg, København, DK (Run 20)
mAb MNF116 Trollhättan, SE (Run 20)
mAb Pan CK, Ab2 Ålesund, NO (Run 20)

Desmin

 mAb D33 Helsingborg, SE (Run 21)  ■  Wien, AT (Run 21)
mAb DE-R-11 Aliso Viejo, USA (Run 21)  ■  Uppsala, SE (Run 21)
mAb ZN-18 Hämeenlinna, FI (Run 21)
E-cadherin (ECAD) mAb 36B5 Mistelbach, AT (Run B5)
mAb ECH-6 Århus, DK (Run B5)
mAb HECD-1 Göteborg, SE (Run B5)
mAb NCH-38 Haarlem, NL (Run B5)

EMA

mAb E29

Falun, SE (Run 10)  ■  Holstebro, DK (Run 10)  ■
Næstved, DK (Run 10)  ■  Tampere, FI (Run 10)

Epithelial antigen (Ep-CAM)

 mAb Ber-EP4 Norbyhagen, NO (Run 23)  ■  Stockholm, SE (Run 23)
mAb MOC-31 Wien, AT (Run 23)
mAb VU-1D9 Apeldoorn, NL (Run 23)

Estrogen receptor

 mAb 1D5 Hjørring, DK (Run B5)
mAb 1D5+ER-2-123 Anonymous (Run B5)
mAb 6F11 Helsinki, FI (Run B5)  ■  Roskilde, DK (Run B5)
rmAb SP1 Roermond, NL (Run B5)  ■  Vejle, DK (Run B5)

Factor VIII related antigen

pAb 760-2642

Oslo, NO (Run 11)

pAb A0082

Lindköping, SE (Run 11)  ■  Skövde, SE (Run 11)  ■
Tampere, FI (Run 11)

Glial fibrillary acidic protein

mAb 6F2

Falun, SE (Run 13)  ■  Karlstad, SE (Run 4)

mAb GA5

Kaunas, LT (Run 13)

pAb Z0334

Anonymous (Run 13)  ■  Seinäjoki, FI (Run 13)
HER-2 pAb A0485 Genk, BE (Run B5)
HercepTest Aliso Viejo, USA (Run B5)
Pathway Castelfranco Veneto, IT (Run B5)

Human chorionic gonadotropin

pAb A231

Esbjerg, DK (Run11)  ■  Holstebro, DK (Run 11)  ■ 
Lund, SE (Run 11)

pAb NCL-HCGp

Firenze, IT (Run 11)
IgK pAb A0191 Jönköping, SE (Run 23)  ■  Odense, DK (Run 23)
IgL pAb A0193 Stockholm, SE (Run 15)  ■  Holstebro, DK (Run 15)
IgM pAb A0425 Bergen, NO (Run 23)  ■  Seinäjoki, FI (Run 23)  ■
Wien, AT (Run 23)
Ki67 mAb K2 's-Hertogenbosch, NL (Run 19)
mAb MIB1 Esbjerg, DK (Run 19)  ■  Trondheim, NO (Run 19)  ■  Århus, DK (Run 19)
mAb MM1 Kiev, UA (Run 19)
rmAb 30-9 Tønsberg, NO (Run 19)
rmAb SP6 Linz, AT (Run 19)  ■  Ålesund, NO (Run 19)
MLA mAb A103 Danderyd, Stockholm, SE (Run 20)  ■ 
Dresden, DE (Run 20)  ■  Wien, AT (Run 20)
mAb A103+M2+7C10+M2-9E3 Helsinki, FI (Run 20)

Mismatch repair proteins MLH1

mAb G168-15

Hillerød, DK (Run 13)  ■  Irvine, USA (Run 13)  ■
Odense, DK (Run 13)  ■  Trondheim, NO (Run 13)

Mismatch repair proteins MSH2

mAb 25D12

 Trondheim, NO (Run 22)

mAb 27

 Roskilde, DK (Run 22)

mAb FE11

 Næstved, DK (Run 22)

MSA-HMB45

mAb HMB45 (HIER)

Firenze, IT (Run 20)  ■  Lillehammer, NO (Run 20)
mAb HMB45 (None) Berlin, DE (Run 20)  ■  Wien, AT (Run 20)
NFP mAb 2F11 Jönköping, SE (Run 18)  ■  Sundsvall, SE (Run 18)
mAb NFP7, DA2, RmdO20.11 Firenze, IT (Run 18)  ■  Oslo, NO (Run 18)

p53

 mAb BP53-12 Barcelona, ES (Run 20)
mAb DO-1 Kassel, DE (Run 20)

mAb DO-7

Örebro, SE (Run 20)  ■  Sundsvall, SE (Run 20)
mAb DO-7+BP53-12 Wien, AT (Run 20)
p63 mAb 4A4 Linköping, SE (Run 16)  ■  Stockholm, SE (Run 16)
mAb 4A4 + Y4A3 Blaricum, NL (Run 16)
mAb BC4A4 Aliso Viejo, USA (Run 16)

Placental alkaline phosphatase

mAb NB10

Firenze, IT (Run 14)

mAb PL8-F6

Sønderborg, DK (Run 14)  ■  Uppsala, SE (Run 14)
Podop mAb D2-40 Leiden, NL (Run 19)  ■  RH, København, DK (Run 19)
PR mAb 16 Roskilde, DK (Run B4)
mAb PGR 636 Wien, AT (Run B4)
rmAb 1E2 Århus, DK (Run B4)
rmAb SP2 Wien, AT (Run B4)

Prostate-specific antigen

mAb 35H9

Aalborg, DK (Run 12)

mAb ER-PR8

Bodø, NO (Run 12)

mAb ER-PR8+PA05

Jyväskylä, FI (Run 12)

pAb 760-2506

Tønsberg, NO (Run 12)

pAb A0562

Karlskrona, SE (Run 12)  ■  Slagelse, DK (Run 12)

Prostatic acid phosphatase

mAb PASE/4LJ

Kristiansand, NO (Run 12)  ■  Seinäjoki, FI (Run 12)

pAb A0627

Helsingborg, SE (Run 12)  ■  Holstebro, DK (Run 12)

S-100

pAb Z0311 (Prot)

Luleå, SE (Run 20)  ■  Västerås, SE (Run 20)
pAb Z0311 (HIER) Wien, AT (Run 20)  ■  Århus, DK (Run 20)
SYP mAb 27G12 Mikkelin, FI (Run 22)
mAb Snp88 Hillerød, DK (Run 22)
pAb 18-0130 Turku, FI (Run 22)
pAb A0010 Örebro, SE (Run 22)
TdT mAb SEN28 Bergen, NO (Run 18)  ■  Sundsvall, SE (Run 18)
pAb A3524 Herlev, DK (Run 18)  ■  Umeå, SE (Run 18)
pAb 004 Leiden, NL (Run 18)

TTF1

mAb SPT24

 Hämeenlinna, FI (Run 23)  ■  Helsingborg, SE (Run 23)  ■  Trondheim, NO (Run 23)

Vimentin

mAb 3B4

Mikkeli, FI (Run 12)

mAb SP20

Ålesund, NO (Run 12)

mAb V9

Esbjerg, DK (Run 12)  ■  Luleå, SE (Run 12)

WT1

 mAb 6F-H2 Vejle, DK (Run 15)  ■  Helsinki, FI (Run 15)
MV/SN/AS

Last update 03-07-2008