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Current protocols
 

Recommended protocols from NordiQC assessment schemes

Among protocols shown to give optimal staining results, one or more are selected to cover a spectrum of laboratories, antibodies, protocols and platforms.

Only the latest recommended protocols for each antibody/clone/epitope are listed here. Previously recommended protocols are not listed any longer but may be obtained from NordiQC on request.

Laboratories producing optimal stains are named in the protocols to encourage direct communication. If a laboratory wishes to remain anonymous, this must be specified when protocols are submitted.
 

mAb = mouse monoclonal antibody, rmAb = rabbit monoclonal antibody, pAb = polyclonal antibody

Protocols Run 37 and B15 (April 2013)

Epitope

Antibody

Platform

Dako

Leica

Ventana

Other
CD3 mAb F7.2.38  CD3-run 37

-

 - CD3-run37
mAb PS1 CD3-run37 CD3-run37 CD3-run37 -
rmAb SP7 CD3-run37 - CD3-run37 -
pAb A0452 CD3-run37 CD3-run37 CD3-run37 -
CD45 mAb 2B11+PD7/26 CD45-run37 CD45-run37 CD45-run37 CD45-run37
mAb X16/99 CD45-run37 CD45-run37 CD45-run37 -
CD56 123C3.D5 CD56-run37 - - -
123C3 CD56-run37 - - -
1B6 - CD56-run37 - -
56C04 - - - CD56-run37
CD564 - - CD56-run37 -
MRQ-42 - - CD56-run37 -
CEA mAb II-7 CEA-run37 CEA-run37 - -
mAb Col-I CEA-run37 CEA-run37 CEA-run37 -
Synaptophysin (SYP) mAb 27G12 SYP-run37 SYP-run37 SYP-run37 SYP-run37
mAb Snp88 SYP-run37 - - -
rmAb SP11 SYP-run37 - SYP-run37 -
HER-2 IHC
rmAb SP3 - HER2-runB15 HER2-runB15 -
pAb A0485 HER2-runB15 - HER2-runB15 -
HercepTest™ (SK001) HER2-runB15 - - -
PATHWAY® (rmAb 4B5) - - HER2-runB15 -
Oracle™ (mAb CB11) - HER2-runB15 - -
Estrogen receptor alpha (ER) 6F11 - - - ER-runB15
EP1 ER-runB15 - ER-runB15 -
SP1 ER-runB15 ER-runB15 ER-runB15 -

Epitope

Antibody

All current protocols

Previous protocols

A        B        C        D        E        F        G        H        K        M      O      P        S        T       V
ALK mAb ALK1 Herlev, DK (Run 17)  ■  Sundsvall, SE (Run 17)
mAb 5A4 Jyväskylä, FI (Run 17)
AMACR mAb 13H4 Anonymous (Run 26)  ■  Hämeenlinna, FI (Run 26)  ■
 Lier, BE (Run 26)  ■  Västerås, SE (Run 26)
pAb CP200C Wien, AT (Run 26)
AMACR/PIN-cocktail cocktail Esbjerg, DK (Run 26)  ■  Kassel, DE (Run 26)  ■
 Slagelse, DK (Run 26)

ASMA

 mAb 1A4 Næstved, DK (Run 27)  ■  Stockholm, SE (Run 27)  ■
Berlin, DE (Run 27)

Bcl-2

mAb 100 

Chemnitz, DE (Run 28)

mAb 100/D5

 Granada, ES (Run 28)

mAb 124

 Hillerød, DK (Run 28)  ■  Odense, DK (Run 28)  ■
Trollhättan, SE (Run 28)

mAb Bcl-2/100/D5

 Oslo, NO (Run 28)

Bcl-6

 mAb LN22 Odense, DK (Run 28)  ■  Jyväskylä, FI (Run 28)
mAb P1F6+PG-B6p Wien, AT (Run 28)
mAb PG-B6p Trondheim, NO (Run 28)  ■  Stockholm, SE (Run 28)
BSAP mAb 1EW Tampere, FI (Run 28)
mAb 24 Tilburg, NL (Run 28)
mAb DAK-Pax5 Herlev, DK (Run 28)
rmAb SP34 Seinäioki, FI (Run 28)
pAb RB-9406 Umeå, SE (Run 28)

Calretinin (CR)

 mAb 5A5 Aalborg, DK (Run 33)
mAb Dak-Calret1 Vilnius, LT (Run 33)
mAb 5A5 Horn, NL (Run 33)
pAb 18-0211 Aliso Viejo, US (Run 33)
CA125 mAb M11 Kalmar, SE (Run 29) ■  Leeuwarden, NL (Run 29) ■
 Wiener Neustadt, AT (Run 29) 
mAb OV185:1 Eindhoven, NL (Run 29) ■  Wien, AT (Run 29)

CD3

 mAb 2GV6 Malmö, SE (Run 22)
mAb F7.2.38 Sarajevo, BA (Run 22)
mAb LN10 Rotterdam, NL (Run 22)

mAb PS1

 Oulu, FI (Run 22)
mAb SP7 Skive, DK (Run 22)

pAb A0452

 Bodø, NO (Run 22)

CD4

 mAb 1F6 Stockholm, SE (Run 29)
mAb 4B12 Linköping, SE (Run 29) ■  Rijeka, HR (Run 29)
mAb SP35 Aalborg, DK (Run 29)

CD5

 mAb 4C7 Rotterdam, NL (Run 34)  ■  Vilnius, LT (Run 34)  ■
Bozen, IT (Run 34)
rmAb SP19 Aalborg, DK (Run 34) ■  Bodø, NO (Run 34)

CD8

mAb 1A5

Irvine, USA (Run 14)

mAb 4B11

Seinäjoki, FI (Run 14)

mAb CD8/144B

Västerås, SE (Run 14)
CD10 mAb 56C6 Leeuwarden, NL (Run 27)  ■  Amsterdam, NL (Run 27)  ■ Göteborg, Se (Run 27)  ■ Anonymous (Run 27)

CD14

mAb 7

Helsinki, FI (Run 11)  ■  Næstved, DK (Run 11)

CD15

mAb C3D-1

 Linköping, SE (Run 25)
mAb Carb-3 Næstved, DK (Run 25)  ■  Barcelona, ES (Run 25)
mAb H198 Kuopio, FI (Run 25)
mAb MMA Roskilde, DK (Run 25)  ■  Anonymous (Run 25)
CD19

mAb LE-CD19

 Aalborg, DK (Run 35)  ■  Næstved, DK (Run 35)  ■
Oulu, FI (Run 35)

CD20

 mAb 7D1 Stockholm, SE (Run 35)
mAb L26 Dublin, IE (Run 35)  ■  Iørenskog, NO (Run 35)
rmAb EP7 Burlingame, US (Run 35)

CD23

mAb 1B12

 Næstved, DK (Run 34)  ■  Rotterdam, NL (Run 34)

rmAb SP23

 Vicenza, IT (Run 34)  ■  Seinäjoki, FI (Run 34)
mAb DAK-CD23 Holstebro, DK (Run 34)

CD30

 mAb 1G12 Aliso Viejo, USA (Run 31)
mAb Ber-H2 Rotterdam, NL (Run 31)  ■  Norbyhagen, N (Run 31)
mAb COND6/B5 Næstved, DK (Run 31)
mAb JCM182 Wien, AT (Run 31) 

CD31

 mAb JC70A Herston, AU (Run 32)  ■  Oslo, N (Run 32)  ■
Skövde, SE (Run 32)

CD34

mAb My10

København, DK (Run 30)

QBEnd 10

Rijeka, HR (Run 30)  ■  Karlskrona, S (Run 30)  ■
Tullamore, IRL (Run 30)

CD45 mAb 2B11+PD7/26 Trondheim, NO (Run 15)  ■  Sundsvall, SE (Run 15)
mAb RP2-18 Irvine, USA (Run 15)
mAb T29/33 Guangzhou, CHN (Run 15)
CD56 mAb 56C04 Hjørring, DK (Run 31)
mAb 1B6 Oslo, N (Run 31)
mAb 123C3 Helsinki, FIN (Run 31)
mAb 123C3.D5 Lillehammer, NO (Run 31)
mAb CD564 Wiener Neustadt, A (Run 31)
rmAb MRQ-42 Odense, DK (Run 31)

CD68

 mAb KP1 Utrecht, NL (Run 31)
mAb PG-M1 Kristianstad, SE (Run 31)  ■  Wien, AT (Run 31)
mAb 514H12 PM, NL (Run 31)

CD79a

 mAb JCB117 Malmö, SE (Run 29) ■  Stockholm, SE (Run 29) ■
 Sv. Nedjelja, HR (Run 29)
rmAb SP18 Bonn, DE (Run 29) ■  Skien, NO (Run 29)

CD99

mAb 12E7

 Vejle, DK (Run 28)  ■  Seinäioki, FI (Run 28)
mAb EPR3097Y Göteborg, SE (Run 28)  ■  Aalborg, DK (Run 28)

CD117

 rmAb YR145 Aalborg, DK (Run 26)  ■  Næstved, DK (Run 26)
pAb A4502 Hillerød, DK (Run 26)  ■  Kassel, DE (Run 26)  ■
 Lahti, FI (Run 26)
CD138 mAb B-A38 Dublin, IE (Run 36)
mAb B-B4 Seinäjoki, FI (Run 36)
mAb MI15 Trondheim, NO (Run 36)  ■  Stockholm, SV (Run 36)
CD163 mAb 10D6 Halmstad, SE (Run 31)
mAb EDhu-1 København, DK (Run 31)
rmAb MRQ-26 Aalborg, DK (Run 31)
CDX2 mAb DAK-CDX2 Blaricum, NL (Run 33)  ■
Santiago de Compostela, E (Run 33)    
rmAb EPR2764Y Holstebro, DK (Run 33)  ■  Lund, S (Run 33)  ■
Roskilde, DK (Run 33)

CEA

mAb II-7

 Helsingborg, SE (Run 27)  ■  Rotterdam, NL (Run 27)  ■
Santiago de Compostela, ES (Run 27)

mAb COL-1

 Odense, DK  ■  Drammen, NO
Chromogranin (CGA) mAb LK2H10 + PHE5 Vancouver, CA (Run 31)
mAb LK2H10 Blaricum, NL (Run 31)
pAb A0430 Danderyd, Stockholm, SE (Run 31) ■
Reykjavik, IS (Run 31)  ■  Turku, FIN (Run 31)
Cyclin D1 rmAb SP4 Bodø, NO (Run 33)  ■  Cork, IRL (Run 33)  ■
Stockholm, SE (Run 33)  ■  Täby, SE (Run 33)
Cytokeratin, high molecular weight mAb D5/16 B4 Herston, AU (Run 32)  ■  Odense, DK (Run 32)
mAb XM26 Aalborg, DK (Run 32)  ■  Herlev, DK (Run 32)  ■
 Roskilde, DK (Run 32)
mAb XM26 & LL002 Chemnitz, D (Run 32)
Cytokeratin, low molecular weight mAb 5D3 Slagelse, DK (Run 33)
mAb B22.1 & B23.1 Aalborg, DK (Run 33)
mAb C51 Saskatoon, CA (Run 33)
mAb CD10 Eindhoven, NL (Run 33)  ■   Randers, DK (Run 33)  ■ Trollhättan, SE (Run 33)
rmAb EP17 Odense C, DK (Run 33)
mAb TS1 Zaandam, NL (Run 33)

Cytokeratin, pan-

 mAb AE1/AE3 Hoorn, NL (Run 36)  ■  Wien, AT (Run 36)  ■
Tønsberg, NO (Run 36)
mAb AE1/AE3 + 5D3 Roskilde, DK (Run 36)
mAb KL1 Vejle, DK (Run 36)

Cytokeratin 5

mAb 34BE12

Irvine, USA (Run 12)  ■  Luleå, SE (Run 12)

mAb D5/16 B4

Seinäjoki, FI (Run 12)  ■  Svendborg, DK (Run 12)

mAb XM26

Kalmar, SE (Run 12)  ■  Stockholm, SE (Run 12)

Cytokeratin 7

mAb OV/TL 12/30

 Seinajoki, FI (Run 25)  ■  Firenze, IT (Run 25)  ■
 Göteborg, SE (Run 25)
Cytokeratin 19 mAb A53-B/A2.26 Wentworthville, AU (Run 34)
mAb B170 Stockholm, SE (Run 34)
mAb BA17 Sønderborg, DK (Run 34)
mAb Ks19.1 Linköping, SE (Run 34)
mAb RCK108 Stavanger, NO (Run 34)

Cytokeratin 20

 mAb Ks 20.8 Alkmaar, NL (Run 35)  ■  Cork, IE (Run 35)  ■
Svendborg, DK (Run 35)
pAb E16444 Breda, NL (Run 35)  ■  Winschoten, NL (Run 35)

Desmin

 mAb D33 Oulu, FI (Run 35)  ■  Vilnius, LT (Run 35)
mAb DE-R-11 Rotterdam, NL (Run 35)  ■  Stockholm, SE (Run 35)
pAb RB-9014 Willemstad, Curacao (Run 35)
E-cadherin (ECAD) mAb 36B5 Mistelbach, AT (Run B5)
mAb ECH-6 Århus, DK (Run B5)
mAb HECD-1 Göteborg, SE (Run B5)
mAb NCH-38 Haarlem, NL (Run B5)

Epithelial membrane antigen (EMA)

mAb E29

 Vancouver, CA (Run 28)  ■  Kristiansand, NO (Run 28)  ■ Eindhoven, NL (Run 28)
mAb GP1.4 Luleå, SE (Run 28)
mAb Mc5 Mistelbach, AT (Run 28)

Epithelial cell adehesion molecule (Ep-CAM)

 mAb Ber EP4 Borås, S (Run 32)  ■  Linköping, S (Run 32)
mAb MOC-31 Lillehammer, N (Run 32)
mAb VU-1D9 Nijmegen, NL (Run 32)  ■  Sønderborg, DK (Run 32)

Estrogen receptor alpha (ER)

 mAb 6F11 London, UK (Run B13)  ■  Oulu, FI (Run B13)
rmAb SP1 Mistelbach, AT (Run B13)  ■  Wiener Neustadt, AT (Run B13)
rmAb EP1 Mumbai, IN (Run B13)

Factor VIII related antigen

pAb 760-2642

Oslo, NO (Run 11)

pAb A0082

Lindköping, SE (Run 11)  ■  Skövde, SE (Run 11)  ■
Tampere, FI (Run 11)

Glial fibrillary acidic protein

mAb 6F2

Falun, SE (Run 13)  ■  Karlstad, SE (Run 4)

mAb GA5

Kaunas, LT (Run 13)

pAb Z0334

Anonymous (Run 13)  ■  Seinäjoki, FI (Run 13)
Gross cystic disease fluid protein-15 mAb 23A3 Aliso Viejo, US (Run 36)  ■  Zagreb, HR (Run 36)
mAb D6 Amersfoort, NL (Run 36)
rmAb EP1582Y Aalborg, DK (Run 36)
Hepatocyte antigen mAb OCH1E5 Næstved, DK (Run36)  ■  Malmö, SE (Run36)  ■
Tilburg, NL (Run36)
HER-2 IHC mAb CB11 Amersfoort, NL (Run B14)
rmAb SP3 Rovaniemi, FI (Run B14)
pAb A0485 Milano, IT (Run B14)
HercepTest™ (SK001) Vienna, AT (Run B14)
PATHWAY® (rmAb 4B5) Stockholm, SE (Run B14)
Oracle™ (mAb CB11) Badalona (Barcelona), ES (Run B14)
HER-2 (Gastric) CONFIRM™ (rmAb 4B5) Turku, FIN (Run G2)
pAb A0485 Leiden, NL (Run G2)
HercepTest™ (K5207) Graz, AT (Run G2)

Human chorionic gonadotropin

pAb A231

Esbjerg, DK (Run11)  ■  Holstebro, DK (Run 11)  ■
Lund, SE (Run 11)

pAb NCL-HCGp

Firenze, IT (Run 11)
IgK pAb A0191 Jönköping, SE (Run 23)  ■  Odense, DK (Run 23)
IgL pAb A0193 Stockholm, SE (Run 15)  ■  Holstebro, DK (Run 15)
IgM pAb 0425 Eindhoven, NL (Run 30)  ■  Norbyhagen Oslo, N (Run 30)  ■ Turku, FIN (Run 30)
Ki-67
mAb MIB1 Lund, SE (Run B13)  ■  Saskatoon, CA (Run B13)
mAb MM1 Bozen, IT (Run B13)
rmAb SP6 Holstebro, DK (Run B13)  ■  Wien, AT (Run B13)
Mammaglobin mAb 304-1A5 Vejle, DK (Run 25)  ■  Göteborg, SE (Run 25)
rmAb 31A5 Skövde, SE (Run 25)
Melan A (MLA) mAb A103 Aalborg, DK (Run 31) ■  Portimão, P (Run 31)  ■
 Bodø, N (Run 31)
mAb HMB45+M2-7C10+M2-9E3+T311 Vienna, A (Run 31)  ■  Turku, FIN  (Run 31)

Mismatch repair protein MLH1

 mAb ES05 Leiden, NL (Run 30)  ■  Wien, A (Run 30)
mAb G168-15 Mistelbach, A (Run 30)  ■  Trondheim, N (Run 30)

Mismatch repair protein MSH2

mAb 25D12

 Trondheim, NO (Run 22)

mAb 27

 Roskilde, DK (Run 22)

mAb FE11

 Næstved, DK (Run 22)
MSH6 mAb 44 Concord, US (Run 32)
rmAb EP49 Næstved, DK (Run 32)  ■  Skive, DK (Run 32)
rmAb EPR3945 Esbjerg, DK (Run 32)

Melanosoma specific antigen (MSA / HMB45)

mAb HMB45 (HIER)

Firenze, IT (Run 20)  ■  Lillehammer, NO (Run 20)
mAb HMB45 (None) Berlin, DE (Run 20)  ■  Wien, AT (Run 20)
MUM1 mAb MUM1p Bozen, I (Run 32)  ■  Odense, DK (Run 32)  ■
 Prague, CZ (Run 32)
Neurofilament proteisn (NFP) mAb 2F11 Jönköping, SE (Run 18)  ■  Sundsvall, SE (Run 18)
mAb NFP7, DA2, RmdO20.11 Firenze, IT (Run 18)  ■  Oslo, NO (Run 18)

OCT3/4

 mAb C-10 Lahti, FI (Run 35)  ■  Vejle, DK (Run 35)  ■
 Vienna, AT (Run 35)
mAb MRQ-10 Dublin, IE (Run 35)  ■  Hjørring, DK (Run 35)
mAb N1NK Vicenza, IT (Run 35)
p16ink4a mAb E6H4 Randers, DK (Run 26)  ■  Stavanger, NO (Run 26)
mAb G175-405 Seinajoki, FI (Run 26)
mAb JC8 Hvidovre, DK (Run 26)
p53 mAb BP53-12 Barcelona, ES (Run 20)
mAb DO-1 Kassel, DE (Run 20)

mAb DO-7

Örebro, SE (Run 20)  ■  Sundsvall, SE (Run 20)
mAb DO-7+BP53-12 Wien, AT (Run 20)
p63 mAb 4A4 Den Haag, NL (Run B8)  ■  Anonymous (Run B8)
mAb 4A4 + Y4A3 Lahti, FI (Run B8)
mAb BC4A4 Bridgewater, CA (Run B8)
PAX8 mAb BC12 Concord, US (Run 34)
pAb 363-15 Aalborg, DK (Run 34)
pAb CP379 Odense, DK (Run 34)  ■  Stavanger, NO (Run 34)

Prostatic acid phosphatase (PAP)

mAb PASE/4LJ

Kristiansand, NO (Run 12)  ■  Seinäjoki, FI (Run 12)

pAb A0627

Helsingborg, SE (Run 12)  ■  Holstebro, DK (Run 12)
Prostein (P501s) mAb 10E3 Svendborg, DK (Run 27)

Placental alkaline phosphatase (PLAP)

mAb 8A9

Hoorn, NL (Run 35)  ■  Malmö, SE (Run 35)  ■
 Zagreb, HR (Run 35)
mAb NB10 Wentworthville, AU (Run 35)
mAb PL8-F6 Tampere, FI (Run 35)
rmAb SP15 Trondheim, NO (Run 35)
Podoplanin mAb D2-40 Wien, AT (Run 36)  ■  Borås, SE (Run 36)  ■
 Oslo, NO  (Run 36)
Progesterone (PR) mAb 16 Charlottetown, CA (Run B14)  ■  Oslo, NO (Run B14)
mAb PgR 636 Romford, GB (Run B14)
rmAb SP2 Évora, PT (Run B14)
rmAb Y85 Taipei, TW (Run B14)
Prostate specific antigen (PSA)

mAb 35H9

 Mistelbach, AT (Run 27)

mAb ER-PR8

 Lillehammer, NO (Run 27)

mAb ER-PR8+PA05

 Ålesund, NO (Run 27)

pAb 760-2506

 Anonymous (Run 27)
pAb A0562 Århus, DK (Run 27)  ■  Blaricum, NL (Run 27)

pAb IR514

 Örebro, SE (Run 27)

S-100 protein

pAb Z0311 (HIER)

Delft, NL (Run 34)  ■  Guetersloh, DE (Run 34)  ■
Tullamore, IE (Run 34)
Smooth muscle myosin heavy chain (SMH) mAb S131 Stockholm, SE (Run B8)
mAb SMMS-1 Odense, DK (Run B8)  ■  Anonymous (Run B8)
Synaptophysin (SYP) mAb 27G12 Hämeenlinna, FI (Run 29) ■  Helsingborg, SE (Run 29) ■ Kristiansand, NO (Run 29) 
mAb Snp88 Holstebro, DK (Run 29) ■  Leeuwarden, NL (Run 29)
rmAb SP11 Rotterdam, NL (Run 29) ■  Seinäjoki, FI (Run 29)
TdT mAb SEN28 Bergen, NO (Run 18)  ■  Sundsvall, SE (Run 18)
pAb A3524 Herlev, DK (Run 18)  ■  Umeå, SE (Run 18)
pAb 004 Leiden, NL (Run 18)

Thyroid transcription factor-1 TTF1)

mAb SPT24

 Graz, A (Run 33)  ■  Hillerød, DK (Run 33)  ■
Vilnius, LT (Run 33)

Vimentin

 mAb 3B4 Kristianstad, S (Run 30)
mAb V9 PM, NL (Run 30)  ■  Romford, UK (Run 30)
rmAb SP20 Prague, CZ (Run 30)

Wilms' tumour-1 (WT1)

 mAb 6F-H2 Aalborg, DK (Run 28)  ■  Heerlen, NL (Run 28)
mAb WT49 PM, NL (Run 28)  ■  Svendborg, DK (Run 28)
MV/SN/LE

Last update 16-04-2013