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Current protocols
 

Recommended protocols from NordiQC assessment schemes

Among protocols shown to give optimal staining results, one or more are selected to cover a spectrum of laboratories, antibodies, protocols and platforms.

Only the latest recommended protocols for each antibody/clone/epitope are listed here. Previously recommended protocols are not listed any longer but may be obtained from NordiQC on request.

Laboratories producing optimal stains are named in the protocols to encourage direct communication. If a laboratory wishes to remain anonymous, this must be specified when protocols are submitted.
 

mAb = mouse monoclonal antibody, rmAb = rabbit monoclonal antibody, pAb = polyclonal antibody

Protocols Run 42 and B18 (December 2014)

Epitope

Antibody

Platform

Dako

Leica

Ventana

Other

Bcl-6 mAb LN22 Bcl-6-run42 Bcl-6-run42 Bcl-6-run42 -
mAb PG-B6p Bcl-6-run42 - - Bcl-6-run42
mAb GI191EA8 - - Bcl-6-run42 -
CD15 mAb Carb-3 CD15-run42 CD15-run42 CD15-run42 CD15-run42
mAb MMA CD15-run42 CD15-run42 CD15-run42 CD15-run42
GLP3 mAb 1G12 GLP3-run42 GLP3-run42 GLP3-run42 GLP3-run42
Melan A (MLA) mAb A103 MLA-run42 MLA-run42 MLA-run42 MLA-run42
mAb M2-7C10 - - - MLA-run42
mAb M2-7C10M2-9E3 - - MLA-run42 -
PAX8 mAb MRQ-50 - PAX8-run42 PAX8-run42 PAX8-run42
mAb BC12 - - - PAX8-run42
mAb IL-150 - - - PAX8-run42
pAb PAX8-run42 - PAX8-run42 -
rmAb ZR-1 - PAX8-run42 PAX8-run42 -
HER-2 IHC
rmAb SP3 - - - HER2-runB18
pAb A0485 - - - HER2-runB18
HercepTest™ (SK001) HER2-runB18 - - -
PATHWAY® (rmAb 4B5) - - HER2-runB18 -
Oracle™ (mAb CB11) - HER2-runB18 - -
mAb CB11 - - - HER2-runB18
Progesterone (PR) mAb 16 PR-runB18 PR-runB18 PR-runB18 -
mAb PgR 636 PR-runB18 PR-runB18 - PR-runB18
mAb PgR1294 PR-runB18 - PR-runB18 -
rmAb EP2 - - - PR-runB18

Epitope

Antibody

All current protocols

Platform

Dako

Leica

Ventana

Other

A     B     C     D     E     F     G     H     I     K     M     N     O     P     S     T     V     W

ALK mAb 5A4 ALK-run39 ALK-run39 ALK-run39 -
rmAb D5F3 ALK-run39 ALK-run39 ALK-run39 -
AMACR mAb 13H4 Lier, BE
(Run 26) Västerås, SE (Run 26)
Hämeenlinna, FI (Run 26) Anonymous (Run 26) -
pAb CP200C Wien, AT
(Run 26)
- - -
AMACR/PIN-cocktail cocktail Esbjerg, DK (Run 26)  - Kassel, DE (Run 26) Slagelse, DK (Run 26)

ASMA

mAb 1A4 Berlin, DE (Run 27) Stockholm, SE (Run 27) - Næstved, DK (Run 27)

Bcl-2

mAb 100 

Chemnitz, DE (Run 28) - - -

mAb 100/D5

- - - Granada, ES (Run 28)

mAb 124

Trollhättan, SE (Run 28) Hillerød, DK (Run 28) Odense, DK (Run 28) -

mAb Bcl-2/100/D5

Oslo, NO
(Run 28)
- Odense, DK (Run 28) -
Bcl-6 mAb LN22 Bcl-6-run42 Bcl-6-run42 Bcl-6-run42 -
mAb PG-B6p Bcl-6-run42 - - Bcl-6-run42
GI191EA8 - - Bcl-6-run42 -
BSAP mAb 1EW - BSAP-run41 - -
mAb 24 BSAP-run41 - - BSAP-run41
mAb DAK-Pax5 BSAP-run41 - BSAP-run41 BSAP-run41
rmAb SP34 - - BSAP-run41 -

Calretinin (CR)

mAb 5A5 - - Aalborg, DK (Run 33) -
mAb Dak-Calret1 Vilnius, LT
(Run 33)
- - -
mAb 5A5 - Horn, NL
(Run 33)
- -
pAb 18-0211 - Aliso Viejo, US (Run 33) - -
CA125 mAb M11 Kalmar, SE
(Run 29)
 
Leeuwarden, NL (Run 29) Wiener Neustadt, AT (Run 29)  -
mAb OV185:1 - Eindhoven, NL (Run 29) Wien, AT
(Run 29)
-

CD3

mAb F7.2.38  CD3-run37 - - CD3-run37

mAb PS1

CD3-run37 CD3-run37 CD3-run37 -
mAb SP7 CD3-run37 - CD3-run37 -

pAb A0452

CD3-run37 CD3-run37 CD3-run37 -

CD4

mAb 1F6 - Stockholm, SE (Run 29) - -
mAb 4B12 Rijeka, HR (Run 29) - - Linköping, SE (Run 29)
mAb SP35 - - Aalborg, DK (Run 29) -

CD5

mAb 4C7 Vilnius, LT
(Run 34)
 
Bozen, IT (Run 34) Rotterdam, NL (Run 34) -
rmAb SP19 Bodø, NO
(Run 34)
- Aalborg, DK (Run 34) -

CD8

mAb 1A5

-

- Irvine, USA (Run 14) -

mAb 4B11

-

- - Seinäjoki, FI (Run 14)

mAb CD8/144B

Västerås, SE (Run 14)

- - -
CD10 mAb 56C6 CD10-run39 CD10-run39 CD10-run39 CD10-run39

CD14

mAb 7

Helsinki, FI (Run 11)

- - Næstved, DK (Run 11)
CD15 mAb Carb-3 CD15-run42 CD15-run42 CD15-run42 CD15-run42
mAb MMA CD15-run42 CD15-run42 CD15-run42 CD15-run42
CD19

mAb LE-CD19

Næstved, DK (Run 35) Oulu, FI
(Run 35)
Aalborg, DK (Run 35)  -

CD20

mAb 7D1 - Stockholm, SE (Run 35) - -
mAb L26 Iørenskog, NO (Run 35) - Dublin, IE (Run 35) -
rmAb EP7 - - - Burlingame, US (Run 35)

CD23

mAb 1B12

Næstved, DK (Run 34)  Rotterdam, NL (Run 34) - -

rmAb SP23

- Vicenza, IT (Run 34) Seinäjoki, FI (Run 34) -
mAb DAK-CD23 Holstebro, DK (Run 34) - - -

CD30

mAb 1G12 - Aliso Viejo, US (Run 31) - -
mAb Ber-H2 Norbyhagen, N (Run 31) - Rotterdam, NL (Run 31) -
mAb COND6/B5 Næstved, DK (Run 31) - - -
mAb JCM182 - Wien, AT
(Run 31) 
- -

CD31

mAb JC70A CD31-run38 CD31-run38 CD31-run38 CD31-run38

CD34

mAb My10

København, DK (Run 30)

- - -

QBEnd 10

Rijeka, HR
(Run 30)

Tullamore, IRL (Run 30) Karlskrona, S (Run 30) -
CD45 mAb 2B11+PD7/26 CD45-run37 CD45-run37 CD45-run37 CD45-run37
mAb X16/99 CD45-run37 CD45-run37 CD45-run37 -
CD56 123C3.D5 CD56-run37 - - -
123C3 CD56-run37 - - -
1B6 - CD56-run37 - -
56C04 - - - CD56-run37
CD564 - - CD56-run37 -
MRQ-42 - - CD56-run37 -

CD68

mAb KP1 - - - Utrecht, NL (Run 31)
mAb PG-M1 Wien, AT (Run 31) - Kristianstad, SE (Run 31) -
mAb 514H12 - PM, NL
(Run 31)
- -

CD79a

mAb JCB117 Sv. Nedjelja, HR (Run 29) Stockholm, SE (Run 29) Malmö, SE (Run 29) -
rmAb SP18 Skien, NO (Run 29) - Bonn, DE (Run 29) -

CD99

mAb 12E7

Vejle, DK
(Run 28)
- Seinäioki, FI (Run 28) -
mAb EPR3097Y Göteborg, SE (Run 28) - Aalborg, DK (Run 28) -

CD117

rmAb YR145 - - Aalborg, DK (Run 26) Næstved, DK (Run 26)
pAb A4502 - Hillerød, DK (Run 26) Kassel, DE (Run 26) Lahti, FI (Run 26)
CD138 mAb B-A38 - - Dublin, IE (Run 36) -
mAb B-B4 - - Seinäjoki, FI (Run 36) -
mAb MI15 Trondheim, NO (Run 36) Stockholm, SV (Run 36) - -
CD163 mAb 10D6 Halmstad, SE (Run 31) - - -
mAb EDhu-1 København, DK (Run 31) - - -
rmAb MRQ-26 - - Aalborg, DK (Run 31) -
CDX2 mAb DAK-CDX2 CDX2-run38 - - -
rmAb EPR2764Y CDX2-run38 CDX2-run38 CDX2-run38 CDX2-run38

CEA

mAb II-7 CEA-run37 CEA-run37 - -
mAb Col-I CEA-run37 CEA-run37 CEA-run37 -
Chromogranin (CGA) mAb LK2H10 + PHE5 - - Vancouver, CA (Run 31) -
mAb LK2H10 - - - Blaricum, NL (Run 31)
pAb A0430 Reykjavik, IS (Run 31)  Danderyd, Stockholm, SE (Run 31) Turku, FIN (Run 31) -
Cyclin D1 rmAb SP4 Bodø, NO (Run 33)  Stockholm, SE (Run 33) Cork, IRL (Run 33) Täby, SE (Run 33)
Cytokeratin, high molecular weight mAb D5/16 B4 CK-HMW-run38 - CK-HMW-run38 -
mAb XM26 CK-HMW-run38 CK-HMW-run38 CK-HMW-run38 -
mAb LL002 - CK-HMW-run38 - CK-HMW-run38
Cytokeratin, low molecular weight mAb 5D3 CK-LMW-run38 CK-LMW-run38 - -
mAb CAM5.2 - - CK-LMW-run38 -
mAb C51 - - - CK-LMW-run38
mAb DC10 CK-LMW-run38 CK-LMW-run38 CK-LMW-run38 -
rmAb EP17 - - CK-LMW-run38 -

Cytokeratin, pan-

mAb AE1/AE3 CK-Pan-run41 CK-Pan-run41 CK-Pan-run41 CK-Pan-run41
mAb BS5 - - - CK-Pan-run41
mAb MNF116 - - CK-Pan-run41 -

Cytokeratin 5

mAb 34BE12

-

- Irvine, USA (Run 12)
Luleå, SE (Run 12)
-

mAb D5/16 B4

Seinäjoki, FI (Run 12)

- - Svendborg, DK (Run 12)

mAb XM26

Kalmar, SE
(Run 12)

- Stockholm, SE (Run 12) -

Cytokeratin 7

mAb OV/TL 12/30

CK7-run40 CK7-run40 CK7-run40 CK7-run40
Cytokeratin 19 mAb A53-B/A2.26 - - Wentworthville, AU (Run 34) -
mAb B170 - Stockholm, SE (Run 34) - -
mAb BA17 - - Sønderborg, DK (Run 34) -
mAb Ks19.1 - - - Linköping, SE (Run 34)
mAb RCK108 Stavanger, NO (Run 34) - - -

Cytokeratin 20

mAb Ks 20.8 - Alkmaar, NL (Run 35) Cork, IE (Run 35) Svendborg, DK (Run 35)
pAb E16444 - - Breda, NL
(Run 35)
Winschoten, NL (Run 35)

Desmin

mAb D33 Vilnius, LT
(Run 35)
Oulu, FI (Run 35) - -
mAb DE-R-11 - Stockholm, SE (Run 35) Rotterdam, NL (Run 35) -
pAb RB-9014 - - - Willemstad, Curacao (Run 35)
E-cadherin (ECAD) mAb HECD-1 ECAD-runB16 ECAD-runB16 - -
mAb NCH-38 ECAD-runB16 ECAD-runB16 ECAD-runB16 -

Epithelial membrane antigen (EMA)

mAb E29

Kristiansand, NO (Run 28) Eindhoven, NL (Run 28) Vancouver, CA (Run 28) -
mAb GP1.4 - - Luleå, SE
(Run 28)
-
mAb Mc5 - Mistelbach, AT (Run 28) - -

Epithelial cell adehesion molecule (Ep-CAM)

mAb Ber EP4 Borås, S
(Run 32)
- - Linköping, S (Run 32)
mAb MOC-31 Lillehammer, N (Run 32) - - -
mAb VU-1D9 - - Sønderborg, DK (Run 32) Nijmegen, NL (Run 32)

Estrogen receptor alpha (ER)

mAb 6F11 - ER-runB17 - ER-runB17
rmAb SP1 ER-runB17 ER-runB17 ER-runB17 -
rmAb EP1 ER-runB17 ER-runB17 - -
mAb 1D5 - - - ER-runB17

Factor VIII related antigen

pAb 760-2642

-

- Oslo, NO
(Run 11)
-

pAb A0082

Lindköping, SE (Run 11)
Tampere, FI (Run 11)

- Skövde, SE (Run 11) -

Glial fibrillary acidic protein

mAb 6F2

Falun, SE
(Run 13)
Karlstad, SE (Run 4)

- - -

mAb GA5

-

- - Kaunas, LT (Run 13)

pAb Z0334

Seinäjoki, FI (Run 13)

- - Anonymous (Run 13) 
GLP3 1G12 GLP3-run42 GLP3-run42 GLP3-run42 GLP3-run42
Gross cystic disease fluid protein-15 mAb 23A3 Zagreb, HR (Run 36) Aliso Viejo, US (Run 36) - -
mAb D6 - - - Amersfoort, NL (Run 36)
rmAb EP1582Y - - Aalborg, DK (Run 36) -
Hepatocyte antigen mAb OCH1E5 Næstved, DK (Run36) - Malmö, SE (Run36) Tilburg, NL (Run36)
HER-2 IHC
rmAb SP3 - - - HER2-runB18
pAb A0485 - - - HER2-runB18
HercepTest™ (SK001) HER2-runB18 - - -
PATHWAY® (rmAb 4B5) - - HER2-runB18 -
Oracle™ (mAb CB11) - HER2-runB18 - -
mAb CB11 - - - HER2-runB18
HER-2 (Gastric) CONFIRM (rmAb 4B5) - - Turku, FIN
(Run G2)
-
pAb A0485 Leiden, NL (Run G2)
HercepTest™ (K5207) Graz, AT
(Run G2)
- - -

Human chorionic gonadotropin

pAb A231

Esbjerg, DK (Run11) Holstebro, DK (Run 11)
Lund, SE
(Run 11)

- - -

pAb NCL-HCGp

Firenze, IT
(Run 11)

- - -
IgK pAb A0191 Jönköping, SE (Run 23) Odense, DK (Run 23) - - -
IgL pAb A0193 Stockholm, SE (Run 15) Holstebro, DK (Run 15) - - -
IgM pAb A0425 IgM-run39 IgM-run39 IgM-run39 -
Ki-67
mAb MIB1 Saskatoon, CA (Run B13) - Lund, SE
(Run B13)
-
mAb MM1 - Bozen, IT (Run B13) - -
rmAb SP6 Holstebro, DK (Run B13) - Wien, AT
(Run B13)
-
Mammaglobin mAb 304-1A5 Vejle, DK (Run 25)
Göteborg, SE (Run 25)
- - -
rmAb 31A5 - - Skövde, SE (Run 25) -
Melan A (MLA) mAb A103 MLA-run42 MLA-run42 MLA-run42 MLA-run42
M2-7C10 - - - MLA-run42
M2-7C10M2-9E3 - - MLA-run42 -

Mismatch repair protein MLH1

mAb ES05 MLH1-run40 MLH1-run40 MLH1-run40 MLH1-run40
mAb G168-15 MLH1-run40 MLH1-run40 MLH1-run40 -

Mismatch repair protein MSH2

mAb FE11

MSH2-run41 MSH2-run41 - -

mAb G219

- - MSH2-run41 -
Mismatch repair protein MSH6 mAb 44 - - - Concord, US (Run 32)
rmAb EP49 Næstved, DK (Run 32) - Skive, DK
(Run 32)
-
rmAb EPR3945 Esbjerg, DK (Run 32) - - -
Mismatch repair protein PMS2 mAb A16-4 PMS2-run41 PMS2-run41 - -
rmAb EP51 PMS2-run41 PMS2-run41 PMS2-run41 -
mAb MOR4G - - - PMS2-run41

Melanosoma specific antigen (MSA / HMB45)

mAb HMB45

MSA-run40 MSA-run40 MSA-run40 MSA-run40
MUM1 mAb MUM1p -
 
Bozen, I
(Run 32)
Odense, DK (Run 32)  Prague, CZ (Run 32)
Napsin A mAb IP64 NapsinA-run39 NapsinA-run39 NapsinA-run39 -
mAb MRQ-60 NapsinA-run39 - - NapsinA-run39
Neurofilament proteisn (NFP) mAb 2F11 Jönköping, SE (Run 18)  - Sundsvall, SE (Run 18) -
mAb NFP7, DA2, RmdO20.11 Oslo, NO (Run 18) - Firenze, IT
(Run 18)
-

OCT3/4

mAb C-10 - Vienna, AT (Run 35) Vejle, DK
(Run 35)
Lahti, FI (Run 35)
mAb MRQ-10 - - Dublin, IE
(Run 35) 
Hjørring, DK (Run 35)
mAb N1NK - Vicenza, IT (Run 35) - -
p16ink4a mAb E6H4 Stavanger, NO (Run 26) - Randers, DK (Run 26)  -
mAb G175-405 - - Seinajoki, FI (Run 26) -
mAb JC8 - - - Hvidovre, DK (Run 26)
p53
mAb CC53 - - p53-run38 -

mAb DO-7

p53-run38 p53-run38 p53-run38 -
mAb DO-7+BP53-12 - - - p53-run38
p57 mAb 25B2 - p57-run41 p57-run41 p57-run41
mAb 57P06(KP10) - p57-run41 - p57-run41
mAb 57P06 p57-run41 - p57-run41 -
mAb KP10 p57-run41 - p57-run41 -
p63 mAb 4A4 - p63-run41 vp63-run41 p63-run41
mAb DAK-p63 p63-run41 - p63-run41 p63-run41
PAX8 MRQ-50 - PAX8-run42 PAX8-run42 PAX8-run42
BC12 - - - PAX8-run42
IL-150 - - - PAX8-run42
pAb PAX8-run42 - PAX8-run42 -
ZR-1 - PAX8-run42 PAX8-run42 -

Prostatic acid phosphatase (PAP)

mAb PASE/4LJ

Kristiansand, NO (Run 12)  Seinäjoki, FI (Run 12)

- - -

pAb A0627

Holstebro, DK (Run 12)

- Helsingborg, SE (Run 12) -
Prostein (P501s) mAb 10E3 - - - Svendborg, DK (Run 27)

Placental alkaline phosphatase (PLAP)

mAb 8A9

Zagreb, HR (Run 35)

Hoorn, NL
(Run 35)
Malmö, SE
(Run 35) 
-
mAb NB10 - - Wentworthville, AU (Run 35) -
mAb PL8-F6 - Tampere, FI (Run 35) - -
rmAb SP15 Trondheim, NO (Run 35) - - -
Podoplanin mAb D2-40 Borås, SE
(Run 36)
 
Wien, AT
(Run 36)
Oslo, NO
(Run 36)
-
Progesterone (PR) mAb 16 PR-runB18 PR-runB18 PR-runB18 -
mAb PgR 636 PR-runB18 PR-runB18 - PR-runB18
mAb PgR1294 PR-runB18 - PR-runB18 -
rmAb EP2 - - - PR-runB18
Prostate specific antigen (PSA)

mAb 35H9

PSA-run40 PSA-run40 PSA-run40 -

pAb A0562

PSA-run40 PSA-run40 PSA-run40 -

S-100 protein

pAb Z0311 (HIER)

Guetersloh, DE (Run 34)
 

Delft, NL
(Run 34)
Tullamore, IE (Run 34) -
Smooth muscle myosin heavy chain (SMH) mAb S131 - Stockholm, SE (Run B8) - -
mAb SMMS-1 Odense, DK (Run B8) - Anonymous (Run B8) -
Synaptophysin (SYP) mAb 27G12 SYP-run37 SYP-run37 SYP-run37 SYP-run37
mAb Snp88 SYP-run37 - - -
rmAb SP11 SYP-run37 - SYP-run37 -
TdT mAb SEN28 TdT-run40 TdT-run40 TdT-run40 -
pAb ILP 0049 - - TdT-run40 TdT-run40

Thyroid transcription factor-1 TTF1)

rmAb SP141

- - TTF1-run39 -
mAb SPT24 TTF1-run39 TTF1-run39 TTF1-run39 TTF1-run39

Vimentin

mAb 3B4 - - Kristianstad, S (Run 30) -
mAb V9 Romford, UK (Run 30) PM, NL
(Run 30) 
- -
rmAb SP20 - - - Prague, CZ (Run 30)

Wilms' tumour-1 (WT1)

mAb 6F-H2 Heerlen, NL (Run 28) - Aalborg, DK (Run 28) -
mAb WT49 - PM, NL
(Run 28)
- Svendborg, DK (Run 28)
MV/SN/LE

Last update 09-12-2014