Prostein (P501S)

Characteristics

Prostein (P501S) is a 553-amino acid protein. It is a prostate-specific marker originally identified by cDNA library subtraction in conjunction with high-throughput microarray screening of prostate carcinomas.
Prostein is localized to the Golgi complex in the cytoplasm of cells and has not been detected in any other normal tissue.

Neoplasms

Prostein is detected in almost all prostate carcinomas. Apart from a few urothelial carcinomas prostein has not been detected in any other tumour type.

Application

In the identification of metastatic prostate adenocarcinoma Prostein is as sensitive as Prostate specific antigen and slightly more specific. As both can fail when staining low differentied prostatic carcinomas, the two Abs should be used in a panel.

Visualization

mAb clone 10E3 works well. HIER is mandatory. An alkaline buffer is preferred.

Recommended control tissue: Normal/hyperplastic prostate and high grade prostate adenocarcinoma with known weak Prostein expression.

Assessments

Run 27 2009

Selected references

Chuang A-Y, DeMarzo AM, Veltri RW, Sharma RB, Bieberich CJ, Epstein JI. Immunohistochemical
differentiation of high-grade prostate carcinoma from urothelial carcinoma. Am J Surg Pathol
2007;31(8):1246-55
 

Kalos M, Askaa J, Hylander BS, Repasky EA, Cai F, Vedvick T, Reed SG, Wright GL Jr, Fanger
Prostein expression is highly restricted to normal and malignant prostate tissues. The Prostate 2004; 60:246
 

Xu J, Kalos M, Stolk JA, Zasloff EJ, Zhang X, Houghton RL, Filho AM, Nolasco M, Badaro R, Reed SG.
Identification and characterization of prostein, a novel prostate-specific protein. Cancer Res. 2001; 61:1563