Myosin, smooth muscle heavy chain (SMH)

Characteristics

Myosin is a cytoplasmic actin based motor protein, about 480 kDa, particularly associated with striated muscle cells, smooth muscle cells, and myoepithelial cells. It consist of 2 heavy chains (HC; 223 kDa) and four light chains (LC). Different isotypes of HC are seen in different cell types. In striated muscle cells, HC can be type I = slow myosin, or type II = fast myosin. In smooth muscle cells and myoepithelial cells, there are also two isotypes. Smooth muscle heavy chain myosin (SMH; 200-209 kDa) is a relatively specific marker for terminal smooth muscle cell differentiation, even though myofibroblasts and other cells show limited expression. Thus, SMH has been demonstrated in endothelial cells of lymph node postcapillary venules, splenic sinus lining cells, and dendritic follicular cells in lymph node germinal centres and splenic B-cell areas .

Neoplasms

SMH can be demonstrated in the majority of smooth muscle cell tumours and and myoepithelial tumours.

 
Application

For the identification of smooth muscle cell tumours and and myoepithelial tumours, SMH should be used in a panel of muscle cell markers.

In neoplastic breast lesions, the myoepithelial cells expressing SMH are usually preserved in papillomas, benign sclerosing changes and in situ ductal carcinoma, while not expressed in invasive ductal carcinoma.
Myoepithelial markers therefore are important tools in the differential diagnosis.  Apart from SMH, alpha-smooth muscle actin (ASMA), S-100 protein, calponin, p63, CD10 and High molecular weight cytokeratins are or have been used as myoepithelial cell markers. SMH in combination with p63 have shown the highest combination of sensitivity and specificity.

Visualization

mAb clone SMMS-1 is widely used and may give good results both in concentrated and ready-to-use formats.
mAb clone S131 seems promising but the experience is scarce. HIER is mandatory. An alkaline buffer is recommended.
Control tissue: Tonsils/lymph node - follicular dendritic cells are low expressing cells and should be used as the critical indicator.

Assessments

Run B8 2009

Selected references

Kalof AN, Tam D, Beatty B, Cooper K. Immunostaining patterns of myoepithelial cells in breast lesions: a comparison of CD10 and smooth muscle myosin heavy chain. J Clin Pathol. 2004 Jun;57(6):625-9.

Moriya T, Kasajima A, Ishida K, Kariya Y, Akahira J, Endoh M, Watanabe M, Sasano H. New trends of immunohistochemistry for making differential diagnosis of breast lesions. Med Mol Morphol. 2006 Mar;39(1):8-13.

Pinkus GS, Warhol MJ, O'Connor EM, Etheridge CL, Fujiwara K. Immunohistochemical localization of smooth muscle myosin in human spleen, lymph node, and other lymphoid tissues. Unique staining patterns in splenic white pulp
and sinuses, lymphoid follicles, and certain vasculature, with ultrastructural correlations. Am J Pathol. 1986 Jun;123(3):440-53.

Werling RW, Hwang H, Yaziji H, Gown AM. Immunohistochemical distinction of invasive from noninvasive breast lesions: a comparative study of p63 versus calponin and smooth muscle myosin heavy chain. Am J Surg Pathol. 2003
Jan;27(1):82-90.